Human IL12 p70 ELISA Kit (ARG80829)

$899.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Human
Tested Application ELISA
Specificity
Target Name IL12 p70
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 2.1 pg/ml
Detection Range
Sample Type Cell culture supernatant, Serum, Plasma (EDTA, citrate, heparin)
Sample Volume 50 ul
Precision

CV: <10%

Full Name interleukin 12A
Alternate Names Check alternative names for the Human IL12 p70 ELISA Kit

Expand

IL12A Elisa kits| IL12B Elisa kits|CLMF p35 Elisa kits|CLMF p40 Elisa kits|Cytotoxic lymphocyte maturation factor 35 kDa subunit Elisa kits|Cytotoxic lymphocyte maturation factor 40 kDa subunit Elisa kits|Interleukin 12 alpha chain Elisa kits|Interleukin-12 beta chain Elisa kits|NK cell stimulatory factor chain 1 Elisa kits|NK cell stimulatory factor chain 2 Elisa kits|NKSF1 Elisa kits|NKSF2 Elisa kits


Application Instructions

 

Assay Time 2 h, 1 h, 10 min (RT)


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Human IL12 p70 ELISA Kit  Download
size

96 wells

Cat No.

ARG80829

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

2.1 pg/ml

Sample type

Cell culture supernatant, Serum, Plasma (EDTA, citrate, heparin)

Sample Volume

50 ul

Precision

CV: <10%

Target name

IL12 p70

Assay time

2 h, 1 h, 10 min (RT)

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

Interleukin-12 (IL-12) is a pleiotropic cytokine, formerly termed cytotoxic lymphocyte maturation factor (CLMF) or natural killer cell stimulatory factor (NKSF), which is produced primarily by stimulated macrophages. It was originally identified as a factor produced by human Epstein-Barr Virus transformed B cell lines. Meanwhile IL-12 has been shown to be a proinflammatory cytokine produced by phagocytic cells, B cells, and other antigen-presenting cells that modulate adaptive immune responses by favoring the generation of T-helper type 1 cells. IL-12 exerts a variety of biological effects on human T and natural killer cells. Apart from promotion of Th1 development and its ability to promote cytolytic activity it mediates some of its physiological activities by acting as a potent inducer of interferon (IFN) gamma production and the stimulation of other cytokines from peripheral blood T and NK cells. IFN-g then enhances the ability of the phagocytic cells to produce IL-12 and other proinflammatory cytokines. Thus, IL-12 induced IFN-g acts in a positive feedback loop that represents an important amplifying mechanism in the inflammatory response to infections. Its role in directing development of a Th1 type immune response from naive T cells demonstrates its critical role in regulation of the immune response and strongly suggests its potential usefulness in cancer therapy. IL-12 is a disulfide-linked heterodimeric cytokine composed of a 35 kDa light chain (p35) and a 40 kDa heavy chain (p40) resulting in the only biologically active 70 kDa (p70) form of IL-12. The p40 subunit can also form a homodimer which has been shown to be able to bind the IL-12 receptor and thus acts as an IL-12 antagonist. Additionally,the p40 subunit has been found to be expressed in a high excess over p70. The critical role of IL-12 in several pathogeneses has been shown. Increased plasma levels were found neurological disorders. Significant elevations were measured in autistic patients, and in multiple sclerosis patients. High levels of IL-12 have also been reported for autoimmune diseases and chronic inflammatory reactions, such as in synovial fluid of patients with osteoarthritis, rheumatoid arthritis and seronegative spondylarthropathies, in Sjogren’s syndrome patients and atherosclerosis. Changes in the expressing levels of IL-12 are reported for a large number of both bacterial and viral infections such as obstructive jaundice, septic shock, infection with Mycobacterium tuberculosis, HIV infection. Interleukin-12 also plays a crucial role in allograft rejection and selected inflammatory skin lesions.

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