Human IL2 ELISA Kit (ARG80830)

$899.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Human
Tested Application ELISA
Specificity
Target Name IL2
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 9.9 pg/ml
Detection Range
Sample Type Serum, Plasma, cell culture supernatant, urine, amniotic fluid, whole blood
Sample Volume 100 ul
Precision

CV: <10%

Full Name interleukin 2
Alternate Names Check alternative names for the Human IL2 ELISA Kit

Expand

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Application Instructions

 

Assay Time 2 h, 1 h 30 min (RT)


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Human IL2 ELISA Kit  Download
size

96 wells

Cat No.

ARG80830

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

9.9 pg/ml

Sample type

Serum, Plasma, cell culture supernatant, urine, amniotic fluid, whole blood

Sample Volume

100 ul

Precision

CV: <10%

Target name

IL2

Assay time

2 h, 1 h 30 min (RT)

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

Interleukin-2 (IL-2) plays a central role in the activation and proliferation of lymphocytes that have been primed by antigens. IL-2 plays a pivotal role in for the expansion of most T-cells, natural killer cells and B-cells during certain phases of their response. IL-2 is a 15 kDa glycoprotein encoded by a single gene located in the q26-28 region of human chromosome 4. The cDNA deduced polypeptide consists of 153 amino acids. IL-2 gene expression is regulated at the transcriptional level by several activation pathways. Antigen-specific proliferation of helper and cytotoxic T-lymphocytes following stimulation is critically dependent on IL-2 expression, secretion, and binding to receptors for IL-2 induced in an autocrine fashion on the surface of T-cells. Apart from its most important role to mediate antigen-specific T-lymphocyte proliferation, IL-2 modulates the expression of interferon-g and major histocompatibility antigens, stimulates proliferation and differentiation of activated B-cells, augments natural killer cell activity and inhibits granulocyte-macrophage colony formation. Alterations in the ability of T-cells to synthesize IL-2 have been observed in physiologic and pathologic states. Because of the central role of IL-2 in immune response, IL-2 turned out to be a very important molecule for diagnostic and therapeutic implications. IL-2 displays antitumoral effects, thus being used in cancer therapy. Monitoring of IL-2 levels in serum provides more detailed insights in several pathological situations such as cancer, infectious diseases, transplant rejection, multiple sclerosis, rheumatoid arthritis, systemic lupus erythematosus and type I diabetes.

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