Human Inflammatory Cytokine multiplex ELISA Kit (IL1 alpha, IL1 beta, IL6, IL8, GM-CSF, IFN-gamma, MCAF and TNF-alpha) (ARG80929)

$1,249.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Human
Tested Application ELISA
Specificity
Target Name Human Inflammatory Cytokine multiplex
Conjugation HRP
Conjugation Note At a wavelength of 450 nm
Sensitivity
Detection Range IL1 alpha: 20.3 – 650 pg/mlIL1 beta: 3.28 – 425 pg/mlIL6: 15.6 – 500 pg/mlIL8: 43.75 – 1400 pg/mlGM-CSF: 18.75 – 600 pg/mlINF-gamma: 31.25 – 1000 pg/mlMCAF: 21.85 – 700 pg/mlTNF-alpha: 43.75 – 1400 pg/ml
Sample Type Cell culture supernatant and other biological samples.
Sample Volume 100 ul
Precision

CV: <10%

Full Name
Alternate Names Check alternative names for the Human Inflammatory Cytokine multiplex ELISA Kit (IL1 alpha, IL1 beta, IL6, IL8, GM-CSF, IFN-gamma, MCAF and TNF-alpha)

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Application Instructions

 

Assay Time 3.5 hour


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Human Inflammatory Cytokine multiplex ELISA Kit (IL1 alpha, IL1 beta, IL6, IL8, GM-CSF, IFN-gamma, MCAF and TNF-alpha)  Download
size

96 wells

Cat No.

ARG80929

Conjugation note

At a wavelength of 450 nm

Detection range

IL1 alpha: 20.3 – 650 pg/mlIL1 beta: 3.28 – 425 pg/mlIL6: 15.6 – 500 pg/mlIL8: 43.75 – 1400 pg/mlGM-CSF: 18.75 – 600 pg/mlINF-gamma: 31.25 – 1000 pg/mlMCAF: 21.85 – 700 pg/mlTNF-alpha: 43.75 – 1400 pg/ml

Sample type

Cell culture supernatant and other biological samples.

Sample Volume

100 ul

Precision

CV: <10%

Target name

Human Inflammatory Cytokine multiplex

Assay time

3.5 hour

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

Inflammation is body’s protective response to foreign subjects, pathogens, tissue damage, autoimmune and other harmful stimuli. It is initiated by production of a cascade of chemicals and cytokines in affected area. These pro-inflammatory mediators result in vasodilation, increased vascular permeability, influx of blood, plasma leakage, neutrophil and macrophage infiltration and activation. Inflammatory reaction plays an important role in limiting foreign substance and engulfing pathogens and tissue debris.Acute inflammation is the initial phase to eliminate invaded foreign substances, pathogens and other harmful stimuli. If the stimuli persist, chronic inflammation will evolve. Progressive tissue destruction and shifting cell types occurs simultaneously during chronic inflammation. The cytokine profiles that regulate the procedure are different depending on the causes, location and progress of the inflammation.

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