Human Inhibin A (heterodimer) ELISA Kit (ARG80847)

$1,099.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Human
Tested Application ELISA
Specificity
Target Name Inhibin A
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 1 pg/ml
Detection Range
Sample Type serum, plasma
Sample Volume 50 ul
Precision

CV: <10%

Full Name inhibin, alpha
Alternate Names Check alternative names for the Human Inhibin A (heterodimer) ELISA Kit

Expand

A inhibin subunit Elisa kits|A inhibin subunit precursor Elisa kits|AW55578 Elisa kits|IHA Elisa kits|inhA Elisa kits|INHA_HUMAN Elisa kits|Inhibin alpha chain Elisa kits|Inhibin alpha chain precursor Elisa kits|Inhibin alpha subunit Elisa kits


Application Instructions

 

Assay Time 3 h, 1 h, 15 min (RT/shaking)


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Human Inhibin A (heterodimer) ELISA Kit  Download
size

96 wells

Cat No.

ARG80847

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

1 pg/ml

Sample type

serum, plasma

Sample Volume

50 ul

Precision

CV: <10%

Target name

Inhibin A

Assay time

3 h, 1 h, 15 min (RT/shaking)

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

Inhibins and activins are glycoproteins, secreted by the gonads, that belong to the transforming growth factor beta family [PMID: 2417121]. They participate in differentiation and growth of diverse cell types. Inhibin inhibits secretion of follicle-stimulating hormone by the pituitary [PMID: 3016724]. Inhibin has two isoforms, A and B, with the same alpha subunit but different beta subunits. Inhibin A is a dimer of alpha and beta A subunits, inhibin B is a dimer of alpha and beta B subunits. Activin A is a dimer of beta A subunits, activin AB is a dimer of beta A and beta B chains. Follistatin is bound to inhibin and activin and indirectly modulates the FSH release. In turn, FSH stimulates inhibin gene expression in the ovarian follicle [PMID: 7813465], probably mediated by cAMP [PMID: 2417121]. The serum levels of inhibin, activin, and follistatin are elevated in pregnant women and decrease after delivery [PMID: 9989858]. Until recently, it was not possible to distinguish between circulating functional dimeric inhibin and free alpha-subunit in the normal human menstrual cycle. However, by using the highly characterized pair of antibodies (anti-inhibin A alpha antibody and anti-inhibin beta A subunit antibody), the two-site sandwich ELISA is found to be able to specifically measure only the dimeric inhibin A.

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