Human LC1 antibody ELISA Kit (ARG80364)

$699.00

Size: 96 wells
Conjugation:
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Human
Tested Application ELISA
Specificity
Target Name LC1 antibody
Conjugation
Conjugation Note
Sensitivity 1 U/ml
Detection Range
Sample Type Serum, Plasma
Sample Volume 10 ul
Precision

CV: <10%

Full Name
Alternate Names Check alternative names for the Human LC1 antibody ELISA Kit

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Application Instructions

 

Assay Time 30, 30, 30 min (RT)


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Human LC1 antibody ELISA Kit  Download
size

96 wells

Cat No.

ARG80364

Sensitivity

1 U/ml

Sample type

Serum, Plasma

Sample Volume

10 ul

Precision

CV: <10%

Target name

LC1 antibody

Assay time

30, 30, 30 min (RT)

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

Anti-LC-1 is used for the quantitative determination of IgG antibodies to formiminotransferase-cyclodeaminase in human serum or plasma for the diagnosis of autoimmune hepatitis (AIH). The group of primary autoimmune liver disease (PAL) comprises AIH, primary biliary cirrhosis (PBC) and primary sclerosing cholangitis (PSC). The clinical picture of PAL is in most cases not different from other chronic liver diseases. About 15% of all cases with chronic liver diseases show an autoimmune pathogenesis. Therefore, after exclusion of infectious etiology especially by viruses, the determination of different autoantibodies is recommended. Patients suffering from AIH show a variety of autoantibodies. Due to the appearance of different antibody specificities classification of AIH into different subgroups is discussed. Type I is characterized by the occurrence of antinuclear antibodies (ANA) and antibodies to smooth muscles (ASMA). ASMA recognize antigenic structure formed by polymeric f-Actin. For type II a high prevalence of antibodies to liver and kidney microsomal antigens (LKM) has been described. LKM1 antibodies recognize epitopes of cytochrome P450 IID6, a 50 kDa cytoplasmic protein found in hepatocytes and proximal tubular kidney cells. LC-1 antibodies are specific for type II hepatitis, too. The respective antigen is formiminotransferase-cyclodeaminase (FTCD) a 58 kDa metabolic enzyme located in the cytosol of liver cells. FTCD is a bifunctional protein that comprises distinct globular domains of formiminotransferase and cyclodeaminase combined by a short linker. Patients with type III autoimmune hepatitis exhibit antibodies to the soluble liver antigen (SLA). This type III is not yet fully accepted as independent subgroup for autoimmune hepatitis by the ¡°International Hepatitis Group¡±. In 10% of AIH cases LC-1 autoantibodies are the only liver-related circulating autoantibodies what underlines the importance of this serological parameter for the differential diagnosis of AIH. Czaja AL. Natural history, clinical features and treatment of autoimmune hepatitis. Semin Liver Dis (1987) 4: 1 ¨C 12 Johnson PJ, McFarlane IA. Meeting Report: International Autoimmune Hepatitis Group. Hepatology (1993) 18: 998 ¨C 1005 Lapierre P, Hajoui O, Homberg JC, Alvarez F: Formiminotransferase cyclodeaminase is an organ-specific autoantigen recognized by sera of patients with autoimmune hepatitis. Gastroenterol (1999) 116: 643-649

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