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Supplier : Arigo Biolaboratories
|Production type||ELISA Kits|
|Tested Reactivity||Arabidopsis, Human|
|Conjugation Note||Substrate: TMB and read at 450 nm|
|Sample Volume||200 ul|
|Full Name||Melatonin direct|
|Alternate Names||Check alternative names for the Human Melatonin ELISA Kit Expand|
|Assay Time||18 h, 3 h (2-8 Celcius degree), 1 h, 30 min (RT/shaker)|
|Storage instruction||Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.|
|Note||Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.|
|User’s manual of Human Melatonin ELISA Kit||Download|
|Cat No.|| |
|Conjugation note|| |
Substrate: TMB and read at 450 nm
|Sample type|| |
|Sample Volume|| |
|Target name|| |
|Assay time|| |
18 h, 3 h (2-8 Celcius degree), 1 h, 30 min (RT/shaker)
|Store at|| |
4 Celcius degree
|Storage instruction|| |
Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Melatonin plays an important role in the synchronisation and characterisation of sleep-wake cycles. Endogenous melatonin is a hormone produced in the pineal gland during the dark phase of the day-night cycle. Its secretion is controlled by an endogenous oscillator that is inhibited by light. Daytime melatonin levels are low or even undetectable and rise in the evening during decreasing light intensity. Endogenous Clock In humans, circadian Melatonin profiles in body fluids (saliva, serum, plasma) provide an excellent marker for the setting of the endogenous clock. Night-time levels peak at around 03:00 h am (acrophase) in most healthy individuals. As a general modulator of human and other vertebrate biorhythm, Melatonin is involved in the timing of functions such as sleep, mood, reproduction and immune system activities. Onset of Melatonin Production Both the intra-individual Melatonin onset and acrophase are very stable. Therefore, it will be sufficient to measure the onset of Melatonin production only, once a whole Melatonin profile has been established. The onset point of melatonin secretion under dim light conditions is called DLMO (Dim Light Melatonin Onset). In contrast to other circadian markers like cortisol, melatonin is the preferred circadian marker because of its robustness even in the presence of various exogenous factors like mental pressure or others.
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