Human Melatonin ELISA Kit (ARG80864)

$999.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Arabidopsis, Human
Tested Application ELISA
Specificity
Target Name Melatonin
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 0.5 pg/ml
Detection Range
Sample Type saliva
Sample Volume 200 ul
Precision

CV: <10%

Full Name Melatonin direct
Alternate Names Check alternative names for the Human Melatonin ELISA Kit

Expand


Application Instructions

 

Assay Time 18 h, 3 h (2-8 Celcius degree), 1 h, 30 min (RT/shaker)


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Human Melatonin ELISA Kit  Download
size

96 wells

Cat No.

ARG80864

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

0.5 pg/ml

Sample type

saliva

Sample Volume

200 ul

Precision

CV: <10%

Target name

Melatonin

Assay time

18 h, 3 h (2-8 Celcius degree), 1 h, 30 min (RT/shaker)

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

Melatonin plays an important role in the synchronisation and characterisation of sleep-wake cycles. Endogenous melatonin is a hormone produced in the pineal gland during the dark phase of the day-night cycle. Its secretion is controlled by an endogenous oscillator that is inhibited by light. Daytime melatonin levels are low or even undetectable and rise in the evening during decreasing light intensity. Endogenous Clock In humans, circadian Melatonin profiles in body fluids (saliva, serum, plasma) provide an excellent marker for the setting of the endogenous clock. Night-time levels peak at around 03:00 h am (acrophase) in most healthy individuals. As a general modulator of human and other vertebrate biorhythm, Melatonin is involved in the timing of functions such as sleep, mood, reproduction and immune system activities. Onset of Melatonin Production Both the intra-individual Melatonin onset and acrophase are very stable. Therefore, it will be sufficient to measure the onset of Melatonin production only, once a whole Melatonin profile has been established. The onset point of melatonin secretion under dim light conditions is called DLMO (Dim Light Melatonin Onset). In contrast to other circadian markers like cortisol, melatonin is the preferred circadian marker because of its robustness even in the presence of various exogenous factors like mental pressure or others.

There are no reviews for this product yet.
By submitting a review, get following benefits:

1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!

view database submit reviews

There is no extra validation for this product yet.
Check other extra validated antibodies below:



Welcome to AntibodyPlus!