Human MIP1 alpha / CCL3 ELISA Kit (ARG80167)

$499.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Human
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human MIP-1beta,MIP-1delta,MIP-3alpha,MIP-3beta
Target Name MIP1 alpha / CCL3
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 15 pg/ml
Detection Range 31.25-2000 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Sample Volume 100 ul
Precision

CV: <10%

Full Name chemokine (C-C motif) ligand 3
Alternate Names Check alternative names for the Human MIP1 alpha / CCL3 ELISA Kit

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Application Instructions

 

Assay Time 4 hours


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Human MIP1 alpha / CCL3 ELISA Kit  Download
size

96 wells

Cat No.

ARG80167

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

15 pg/ml

Detection range

31.25-2000 pg/ml

Sample type

Serum, Plasma, Cell culture supernatants

Sample Volume

100 ul

Precision

CV: <10%

Target name

MIP1 alpha / CCL3

Assay time

4 hours

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

MIP1 alpha belongs to the chemokine beta family. In vitro MIP1 alpha stimulates H2O2 production in human neutrophils. MIP1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. Has adhesive effects on lymphocytes. MIP1 alpha can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. A signal transducing receptor, designated the C-C chemokine receptor 1 (C-C CKR-1) with seven transmembrane domains that bind MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities, has been isolated.

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