Human Neopterin ELISA Kit (ARG80878)

$699.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Human
Tested Application ELISA
Specificity
Target Name Neopterin
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 0.7 nmol/l
Detection Range
Sample Type serum, plasma, urine
Sample Volume 20 ul
Precision

CV: <10%

Full Name Neopterin
Alternate Names Check alternative names for the Human Neopterin ELISA Kit

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Application Instructions

 

Assay Time 90 min (RT/shaker/dark), 10 min (RT)


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Human Neopterin ELISA Kit  Download
size

96 wells

Cat No.

ARG80878

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

0.7 nmol/l

Sample type

serum, plasma, urine

Sample Volume

20 ul

Precision

CV: <10%

Target name

Neopterin

Assay time

90 min (RT/shaker/dark), 10 min (RT)

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

Neopterin is a low molecular weight molecule belonging to the chemical group known as pteridines. It is synthesised by cellular immune reaction of macrophages and dendritic cells upon stimulation with the cytokine interferon-g and as a consequence released. Neopterin has a higher stability in body fluids which makes the sample handling and measurement easier compared to other cytokines. The low molecular weight, let neopterin molecules rapidly pass the intravasal area, where it is releases in urine after glomerular filtration. The half life period in human bodies is only affected by renal excretion. So neopterin values reflect the totality of immunological processes for monocytes/macrophages and dendritic cells and can be seen as a general marker of immune activity. This characteristic feature of neopterin to reflect the different interactions of immunocompetent cells is the basis for the extraordinary status of measuring neopterin in immmunological diagnosis. As a non-invasive method, urinary neopterin to creatinine ratio determination is also helpful in monitoring disease progression and the effects of therapies, as well. Neopterin biosynthesis is closely associated with activation of the cellular immune system. Increased concentrations of neopterin were reported in patients with viral infections, suggesting that increased values may originate from the immune response of patients directed against virally infected cells. It was shown that antigenic stimulation of human peripheral blood mononuclear cells leads to neopterin release into cell culture medium and that human macrophages produce neopterin in vitro when stimulated by interferon gamma. The determination of neopterin levels in human body fluids offers a useful and innovative tool to monitor diseases associated with the activation of cell-mediated immunity. Increasing neopterin levels in various infections precede the clinical manifestation and seroconversion. Normally samples are not tested for all possible infections. Therefore, the measurement of neopterin in blood donor samples is a useful tool in order to reduce the risk of infections via blood transfusion.

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