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Supplier : Arigo Biolaboratories
|Production type||ELISA Kits|
|Target Name||Plasma Renin Activity|
|Conjugation Note||Substrate: TMB and read at 450 nm|
|Sample Volume||50 ul|
|Alternate Names||Check alternative names for the Human Plasma Renin Activity (PRA) ELISA Kit Expand
Angiotensin forming enzyme Elisa kits|Angiotensin forming enzyme precursor Elisa kits|Angiotensinogenase Elisa kits|Angiotensinogenase precursor Elisa kits|FLJ10761 Elisa kits|HNFJ2 Elisa kits|REN Elisa kits|Ren1 Elisa kits|RENI_HUMAN Elisa kits|Renin Elisa kits|Renin precursor renal Elisa kits
|Assay Time||1 h, 30, 10-15 min (RT/shaker)|
|Storage instruction||Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.|
|Note||Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.|
|User’s manual of Human Plasma Renin Activity (PRA) ELISA Kit||Download|
|Cat No.|| |
|Conjugation note|| |
Substrate: TMB and read at 450 nm
|Sample type|| |
|Sample Volume|| |
|Target name|| |
Plasma Renin Activity
|Assay time|| |
1 h, 30, 10-15 min (RT/shaker)
|Store at|| |
4 Celcius degree
|Storage instruction|| |
Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
This kit measures PRA and the results are expressed in terms of mass of angiotensin-I (Ang-I) generated per volume of human plasma in unit time (ng/mL.h). Measurement of PRA is important for the clinical evaluation of hypertensive patients. In particular, determination of plasma renin activity can help in the diagnosis of primary hyperaldosteronism (5-13% of hypertensive cases) and assist in the therapy and management of other forms of hypertension. PRA, in contrast to the determination of renin concentration, is a more accurate indicator of primary hyperaldosteronism (PHA), because of several reasons: 1. PRA is the expression of the rate of Ang-I formation through the enzymatic action of renin on its substrate, angiotensinogen, therefore PRA depends not only on renin concentration but also on the concentration of angiotensinogen which is ignored in the renin concentration assay; 2. Plasma renin concentration assay does not ensure sensitivity in low renin states, while the sensitivity of the PRA assay can be enhanced by increasing the incubation time during the generation step (Sealey et al., 2005), 3. When an inhibitor is bound to the renin active site PRA is inhibited, whereas the presence of the inhibitor does not affect the recognition of renin by currently available immunoassays, therefore total renin concentration does not always correlate with plasma renin activity (Campbell et al., 2009). Renin liberates angiotensin-I from angiotensinogen. Angiotensin-I is transformed to angiotensin-II largely in pulmonary circulation by angiotensin converting enzyme (ACE). Angiotensin-II raises blood pressure by direct arteriolar vasoconstriction, promoting sodium retension, and stimulating the secretion of aldosterone from the adrenal cortex. Aldosterone also exerts an effect to restore sodium balance and lift arterial pressure. Accurate measurement of the concentration of circulating angiotensin-II is challenging because of its instability in blood samples. Aldosterone concentration can be easily determined using the immunoassay kit.
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