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Supplier : Arigo Biolaboratories
|Production type||ELISA Kits|
|Conjugation Note||Substrate: TMB and read at 450 nm|
|Sample Type||serum, plasma|
|Sample Volume||100 ul|
|Alternate Names||Check alternative names for the Human Proinsulin ELISA Kit Expand
IDDM Elisa kits|IDDM1 Elisa kits|IDDM2 Elisa kits|ILPR Elisa kits|ins Elisa kits|INS_HUMAN Elisa kits|Insulin A chain Elisa kits|Insulin B chain Elisa kits|IRDN Elisa kits|MODY10 Elisa kits|Preproinsulin Elisa kits|Proinsulin Elisa kits|Proinsulin precursor Elisa kits
|Assay Time||16 – 24 h (4 Celcius degree), 60, 30 min (RT)|
|Storage instruction||Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.|
|Note||Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.|
|User’s manual of Human Proinsulin ELISA Kit||Download|
|Cat No.|| |
|Conjugation note|| |
Substrate: TMB and read at 450 nm
|Sample type|| |
|Sample Volume|| |
|Target name|| |
|Assay time|| |
16 – 24 h (4 Celcius degree), 60, 30 min (RT)
|Store at|| |
4 Celcius degree
|Storage instruction|| |
Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
The measurement of Proinsulin in serum can provide valuable information for the diagnosis of insulinomas. Proinsulin levels have also been shown to be elevated in non-insulin dependent diabetics (NIDDM), newly diagnosed insulin dependent diabetics (IDDM) and other clinical situations. Proinsulin is a 9390 MW polypeptide of 86 amino acids, that is synthesized in the ? cells of the pancreas and is the precursor molecule for insulin. Most proinsulin is converted to insulin and C-Peptide, which are secreted in equimolar amounts into the blood. About 15 % is not converted and is released as proinsulin. The biological activity of proinsulin is only about 10% of Insulin, but the half life of proinsulin is three times as long as insulin. The level of proinsulin in serum can be a reflection of ? cell status. Both IDDM and NIDDM are characterized by dysfunction of the pancreatic ? cells. Elevated proinsulin levels have been noted at the onset of IDDM and in healthy siblings of IDDM patients. Proinsulin levels may also be increased in patients with established NIDDM. Increased levels of circulating proinsulin are found in older patients, pregnant or obese diabetics, patients with insulinomas, functional hypoglycemia and hyperinsulinemia, a rare syndrome. Because the structure of proinsulin is similar to insulin, proinsulin may be detected as immunoreactive insulin in the insulin assay. Immunoreactive insulin levels are generally determined in conventional RIA’s, which overestimate the insulin level because the methods use antibodies, which crossreact with proinsulin. By calculating the molar ration of proinsulin to true insulin (P/I), a better assessment of ? cell function can be made.
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