Human Proinsulin ELISA Kit (ARG80783)

$699.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Human
Tested Application ELISA
Specificity
Target Name Proinsulin
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 0.5 pmol/l
Detection Range
Sample Type serum, plasma
Sample Volume 100 ul
Precision

CV: <10%

Full Name insulin
Alternate Names Check alternative names for the Human Proinsulin ELISA Kit

Expand

IDDM Elisa kits|IDDM1 Elisa kits|IDDM2 Elisa kits|ILPR Elisa kits|ins Elisa kits|INS_HUMAN Elisa kits|Insulin A chain Elisa kits|Insulin B chain Elisa kits|IRDN Elisa kits|MODY10 Elisa kits|Preproinsulin Elisa kits|Proinsulin Elisa kits|Proinsulin precursor Elisa kits


Application Instructions

 

Assay Time 16 – 24 h (4 Celcius degree), 60, 30 min (RT)


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Human Proinsulin ELISA Kit  Download
size

96 wells

Cat No.

ARG80783

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

0.5 pmol/l

Sample type

serum, plasma

Sample Volume

100 ul

Precision

CV: <10%

Target name

Proinsulin

Assay time

16 – 24 h (4 Celcius degree), 60, 30 min (RT)

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

The measurement of Proinsulin in serum can provide valuable information for the diagnosis of insulinomas. Proinsulin levels have also been shown to be elevated in non-insulin dependent diabetics (NIDDM), newly diagnosed insulin dependent diabetics (IDDM) and other clinical situations. Proinsulin is a 9390 MW polypeptide of 86 amino acids, that is synthesized in the ? cells of the pancreas and is the precursor molecule for insulin. Most proinsulin is converted to insulin and C-Peptide, which are secreted in equimolar amounts into the blood. About 15 % is not converted and is released as proinsulin. The biological activity of proinsulin is only about 10% of Insulin, but the half life of proinsulin is three times as long as insulin. The level of proinsulin in serum can be a reflection of ? cell status. Both IDDM and NIDDM are characterized by dysfunction of the pancreatic ? cells. Elevated proinsulin levels have been noted at the onset of IDDM and in healthy siblings of IDDM patients. Proinsulin levels may also be increased in patients with established NIDDM. Increased levels of circulating proinsulin are found in older patients, pregnant or obese diabetics, patients with insulinomas, functional hypoglycemia and hyperinsulinemia, a rare syndrome. Because the structure of proinsulin is similar to insulin, proinsulin may be detected as immunoreactive insulin in the insulin assay. Immunoreactive insulin levels are generally determined in conventional RIA’s, which overestimate the insulin level because the methods use antibodies, which crossreact with proinsulin. By calculating the molar ration of proinsulin to true insulin (P/I), a better assessment of ? cell function can be made.

There are no reviews for this product yet.
By submitting a review, get following benefits:

1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!

view database submit reviews

There is no extra validation for this product yet.
Check other extra validated antibodies below:



Welcome to AntibodyPlus!