Human Prolactin ELISA Kit (ARG80852)

$499.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Human
Tested Application ELISA
Specificity This ELISA kit is no cross-reactivity with hCG, TSH, LH, FSH, or hGH.
Target Name Prolactin
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 0.35 ng/ml
Detection Range
Sample Type serum
Sample Volume 25 ul
Precision

CV: <10%

Full Name prolactin
Alternate Names Check alternative names for the Human Prolactin ELISA Kit

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Application Instructions

 

Assay Time 30, 10 min (RT)


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Human Prolactin ELISA Kit  Download
size

96 wells

Cat No.

ARG80852

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

0.35 ng/ml

Sample type

serum

Sample Volume

25 ul

Precision

CV: <10%

Target name

Prolactin

Assay time

30, 10 min (RT)

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

Human prolactin (lactogenic hormone) is secreted from the anterior pituitary gland in both men and women. Human prolactin is a single chain polypeptide hormone with a molecular weight of approximately 23.000 daltons. The release and synthesis of prolactin is under neuroendocrinal control, primarily through Prolactin Releasing Factor and Prolactin Inhibiting Factor. Women normally have slightly higher basal prolactin levels than men; apparently, there is an estrogen- related rise at puberty and a corresponding decrease at menopause. The primary functions of prolactin are to initiate breast development and to maintain lactation. Prolactin also suppresses gonadal function. During pregnancy, prolactin levels increase progressively to between 10 and 20 times normal values, declining to non-pregnant levels by 3-4 weeks post- partum. Breast feeding mothers maintain high levels of prolactin, and it may take several months for serum concentrations to return to non-pregnant levels. The determination of prolactin concentration is helpful in diagnosing hypothalamic- pituitary disorders. Microadenomas (small pituitary tumors) may cause hyperprolactinemia, which is sometimes associated with male impotence. High prolactin levels are commonly associated with galactorrhea and amenorrhea. Prolactin concentrations have been shown to be increased by estrogens, thyrotropin- releasing hormone (TRH), and several drugs affecting dopaminergic mechanisms. Prolactin levels are elevated in renal disease and hypothyroidism, and in some situations of stress, exercise, and hypoglycemia. Additionally, the release of prolactin is episodic and demonstrates diurnal variation. Mildly elevated prolactin concentrations should be evaluated taking these considerations into account. Prolactin concentrations may also be increased by drugs such as chloropromazine and reserpine, and may be lowered by bromocyptine and L-dopa.

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