Human Resistin ELISA Kit (ARG80885)

$849.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Human
Tested Application ELISA
Specificity
Target Name Resistin
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 0.006 ng/ml
Detection Range
Sample Type Serum, Plasma, cell culture medium, breast milk, saliva, urine
Sample Volume 100 ul
Precision

CV: <10%

Full Name resistin
Alternate Names Check alternative names for the Human Resistin ELISA Kit

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Application Instructions

 

Assay Time 2 h, 1 h, 30 min, 30 min


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Human Resistin ELISA Kit  Download
size

96 wells

Cat No.

ARG80885

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

0.006 ng/ml

Sample type

Serum, Plasma, cell culture medium, breast milk, saliva, urine

Sample Volume

100 ul

Precision

CV: <10%

Target name

Resistin

Assay time

2 h, 1 h, 30 min, 30 min

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

Resistin, a cysteine-rich protein of 11.3 kDa, was firstly found in mice and constitutes together with RELMa, RELMb and RELMg the protein family of resistin-like molecules (RELM). In humans, resistin and RELMb but no other proteins of the RELM family were found. The human form of resistin shows a homology of 53% to the murine protein. It has 11 cysteine-residues, is synthesized as a propetide of 108 amino acids and secreted as a dimer, build by a disulfide bridge of cysteine residues. Beside this intermolecular disulfide bridge, 5 additional intramolecular ones exist. Appearance of multi- and oligomer formation was proved by size exclusion chromatography. Thereby it was shown, that oligomer formation is SDS-insensitive but can be inhibited by ?-mercaptoethanol and is therefore likely to be caused by disulfide bridges. Further on, the resistin structure seems to be dependent on its concentration, as circular dichroism analysis shows a concentration dependent shift of a-helical to b-sheet structure. Resistin expression was demonstrated in white adipose tissue, pituitary and pancreatic islets of mice as well as in brown adipose tissue of rats. In humans, resistin expression in adipocytes can be detected but only at a very low level. But in vitro, resistin expression of non-adipocytes in fatty tissue was shown. Human resistin gene is also expressed in pancreatic islets , pre-adipocytes marcophages and bone marrow. So, resistin is of relevance for inflammation processes as well as for lipid metabolism.

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