Human SCF ELISA Kit (ARG80159)

$499.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Human
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human CNTF, EGF, Epo, G-CSF, GM-CSF, IL-1 alpha, IL-1 beta,2,3,4,5,6,7,8, LIF, SCFR, TGF- beta1, TNF- alpha
Target Name SCF
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 15 pg/ml
Detection Range 31.25-2000 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Sample Volume 100 ul
Precision

CV: <10%

Full Name KIT ligand
Alternate Names Check alternative names for the Human SCF ELISA Kit

Expand

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Application Instructions

 

Assay Time 4 hours


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Human SCF ELISA Kit  Download
size

96 wells

Cat No.

ARG80159

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

15 pg/ml

Detection range

31.25-2000 pg/ml

Sample type

Serum, Plasma, Cell culture supernatants

Sample Volume

100 ul

Precision

CV: <10%

Target name

SCF

Assay time

4 hours

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

Stem Cell Factor (SCF), also called c-kit Ligand or Mast Cell Growth Factor (MGF), is a peptide growth factor/cytokine with broad activities, especially related to hematopoiesis (ref 1-3). SCF acts on early hematopoietic progenitor/stem cells and stimulates the proliferation and survival of mast cells. Also, SCF is one of the most potent stimulators of multi-lineage progenitors (CFU-GEMM) in both human and murine bone marrow cells (ref 2,4). SCF acts synergistically with other growth factors, including erythropoietin, G-CSF, M-CSF, GM-CSF, IL-3 and IL-6, to increase the number and size of colonies of hematopoietic progenitors (ref 1,2,5). SCF appears to play an important role in the survival, proliferation or migration of primordial germ cells and melanoblasts during both development and maturation (ref 6,7).

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