Human SHBG ELISA Kit (ARG80853)

$499.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Human
Tested Application ELISA
Specificity
Target Name SHBG
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 0.2 nmol/l
Detection Range
Sample Type serum, plasma
Sample Volume 25 ul
Precision

CV: <10%

Full Name sex hormone-binding globulin
Alternate Names Check alternative names for the Human SHBG ELISA Kit

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Application Instructions

 

Assay Time 30, 15, 12 min


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Human SHBG ELISA Kit  Download
size

96 wells

Cat No.

ARG80853

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

0.2 nmol/l

Sample type

serum, plasma

Sample Volume

25 ul

Precision

CV: <10%

Target name

SHBG

Assay time

30, 15, 12 min

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

Sex-hormone-binding globulin (SHBG) is a B-globulin that specifically binds steroid hormones. The major site of SHBG synthesis is thought to be the hepatocytes. Its production is regulated by androgen/estrogen balance, thyroid hormones, insulin and dietary factors, among others. SHBG is involved in the transport of sex steroids in plasma. Its concentration is a major factor regulating their distribution between protein-bound and free states. Determination of SHBG concentration is mainly of importance in the evaluation of mild disorders of androgen metabolism and it allows identification of women with hirsutism who are likely to respond to estrogen therapy. Testosterone/SHBG-ratios correlate well with both measured and calculated values for free testosterone, and help to discriminate between subjects with excessive androgen activity and normal individuals. In general the measurement of SHBG offers the possibility for calculating the free fraction of vatious steroid hormones based on the measurement of the total concentration in serum. But there is also a method available for the direct measurement of free steroid hormones in saliva. The metabolic activity of any steroid in any case is just represented by the free hormone fraction which represents less than 5% of the total. The non-invasive sampling of saliva samples offers another advantage of salivary testing.

Database link

All research areas>Signaling Intermediates>SHBG
(View all antibody categories related to Signaling Intermediates)

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