Human Testosterone ELISA Kit (ARG80854)

$499.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Human
Tested Application ELISA
Specificity
Target Name Testosterone
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 0.083 ng/ml
Detection Range
Sample Type serum, plasma
Sample Volume 25 ul
Precision

CV: <10%

Full Name Testosterone
Alternate Names Check alternative names for the Human Testosterone ELISA Kit

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Application Instructions

 

Assay Time 60, 15 min


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Human Testosterone ELISA Kit  Download
size

96 wells

Cat No.

ARG80854

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

0.083 ng/ml

Sample type

serum, plasma

Sample Volume

25 ul

Precision

CV: <10%

Target name

Testosterone

Assay time

60, 15 min

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

Testosterone (17beta-hydroxy-4-androstene-3-one) is a C19 steroid with an unsaturated bond between C-4 and C-5, a ketone group in C-3 and a hydroxyl group in the beta position at C-17. This steroid hormone has a molecular weight of 288.47. Testosterone is the most important androgen secreted into the blood. In males, testosterone is secreted primarily by the Leydig cells of the testes; in females ca. 50% of circulating testosterone is derived from peripheral conversion of androstenedione, ca. 25% from the ovary and ca. 25% from the adrenal glands. Testosterone is responsible for the development of secondary male sex characteristics and its measurements are helpful in evaluating the hypogonadal states. In women, high levels of testosterone are generally found in hirsutism and virilization, polycystic ovaries, ovarian tumors, adrenal tumors and adrenal hyperplasia. In men, high levels of testosterone are associated to the hypothalamic pituitary unit diseases, testicular tumors, congenital adrenal hyperplasia and prostate cancer. Low levels of testosterone can be found in patients with the following diseases: Hypopituitarism, Klinefelter’s syndrome, Testicular feminization, Orchidectomy and Cryptorrchidism, enzymatic defects and some autoimmune diseases.

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