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Supplier : Arigo Biolaboratories
|Production type||ELISA Kits|
|Conjugation Note||Substrate: TMB and read at 450 nm|
|Sample Type||serum, plasma|
|Sample Volume||20 ul|
|Alternate Names||Check alternative names for the Human Testosterone (free) ELISA Kit Expand|
|Assay Time||1 h (37 Celcius degree), 15 min (RT/dark)|
|Storage instruction||Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.|
|Note||Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.|
|User’s manual of Human Testosterone (free) ELISA Kit||Download|
|Cat No.|| |
|Conjugation note|| |
Substrate: TMB and read at 450 nm
|Sample type|| |
|Sample Volume|| |
|Target name|| |
|Assay time|| |
1 h (37 Celcius degree), 15 min (RT/dark)
|Store at|| |
4 Celcius degree
|Storage instruction|| |
Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Testosterone is a steroid hormone from the androgen group. Testosterone is primarily secreted in the testes of males and the ovaries of females although small amounts are secreted by the adrenal glands. It is the principal male sex hormone and an anabolic steroid. In both males and females, it plays key roles in health and well-being. Due to its insolubility in aqueous solutions, for the most part Testosterone circulates in the blood bound to transport proteins. Only a small percentage (< 1%) of circulating Testosterone exists as unbound or free Testosterone. The majority, approximately 60%, is bound to SHBG with high affinity, while the remainder is loosely bound to albumin. Both the albumin-bound and free fractions may be biologically active, while SHBG effectively inhibits Testosterone action. Testosterone effects can be classified as virilizing and anabolic effects. Anabolic effects include growth of muscle mass and strength, increased bone density and strength, and stimulation of linear growth and bone maturation. Virilizing effects include maturation of the sex organs. Testosterone levels decline gradually with age in men. Measurement of the free or unbound fraction of serum Testosterone has been proposed as a means of estimating the physiologically bioactive hormone. Free Testosterone levels are elevated in women with hyperandrogenism associated with hirsutism in the presence or absence of polycystic ovarian disease. In addition, free Testosterone measurements may be more useful than total Testosterone in situations where SHBG is increased or decreased (e.g. hypothyroidism and obesity).
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