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Supplier : Arigo Biolaboratories
|Production type||ELISA Kits|
|Target Name||Thyroid Peroxidase (TPO) autoantibody|
|Conjugation Note||Substrate: TMB and read at 450 nm|
|Sample Type||serum, plasma 1:100 diluted|
|Sample Volume||10 ul|
|Full Name||Thyreoidal Peroxidase Autoantibodies (TPO-Ab)|
|Alternate Names||Check alternative names for the Human Thyroid Peroxidase (TPO) autoantibodies ELISA Kit Expand|
|Assay Time||30, 15, 15, 5 min (RT)|
|Storage instruction||Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.|
|Note||Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.|
|User’s manual of Human Thyroid Peroxidase (TPO) autoantibodies ELISA Kit||Download|
|Cat No.|| |
|Conjugation note|| |
Substrate: TMB and read at 450 nm
|Sample type|| |
serum, plasma 1:100 diluted
|Sample Volume|| |
|Target name|| |
Thyroid Peroxidase (TPO) autoantibody
|Assay time|| |
30, 15, 15, 5 min (RT)
|Store at|| |
4 Celcius degree
|Storage instruction|| |
Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Thyroid disorders are the most prevalent of all autoimmune diseases. Thyroid autoimmune diseases are associated with the occurrence of differentiated autoantibodies and are thought to be related to a genetical pre-disposition. These autoantibodies are directed against membrane- located and/or extracellular antigens of the thyroid cells: Human Thyroglobulin (hTG), a water soluble glycoprotein with a molecular weight of approx. 660.000 Dalton, is the principal constituent of the thyroidal colloid sharing about 75 % of its mass. Synthesis of the thyroid hormones T3 and T4 is based on the oxidative iodination of tyrosine residues of the thyroglobulin molecule. Within the cell thyroglobulin is transported by the microsomes. Together with the secretion of T3 and T4 also small amounts of hTG are liberated into circulation. The microsomal antigen of the thyroid is an integral membrane protein of the microsomes. It has been characterised as the enzyme Thyroid Peroxidase (TPO) with a molecular weight of nearly 110.000 Dalton. The TSH-Receptor is a regulatory protein embedded into the thyroid cell membrane effecting synthesis and release of the thyroid hormones as well as cellular growth. The so-called Colloid-Antigen 2 CA2.
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