Human total IgE ELISA Kit (ARG80774)

$499.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Human
Tested Application ELISA
Specificity
Target Name Human total IgE
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 0.8 IU/ml
Detection Range
Sample Type serum, plasma
Sample Volume 10 ul
Precision

CV: <10%

Full Name Human total IgE
Alternate Names Check alternative names for the Human total IgE ELISA Kit

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Application Instructions

 

Assay Time 30, 15 min


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Human total IgE ELISA Kit  Download
size

96 wells

Cat No.

ARG80774

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

0.8 IU/ml

Sample type

serum, plasma

Sample Volume

10 ul

Precision

CV: <10%

Target name

Human total IgE

Assay time

30, 15 min

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

The existence of IgE in man as a unique class of immunoglobulins which are important in the mediation of the allergic response has been known for over twenty years. The mechanism of action involves an initial antigenic stimulation of immunocompetent B lymphocytes by a specific antigen, a process which induces the lymphocyte to respond by producing specific antibody of several classes. One class, reaginic or IgE antibody, becomes partially bound via its Fc portion to receptors on the surface of mast cells and basophilic leukocytes. Upon further stimulation by specific allergens, these cell-bound IgE molecules bind via their Fab portion to the allergen. This combination triggers the mast cells and basophilic leucocytes to release various vasoactive amines into the blood and the surrounding tissue. These substances cause smooth muscle constriction and lead ultimately to allergic conditions such as wheal and flare reactions, hives, dermatitis, rhinitis, hay fever, asthma and anaphylactic shock. IgE determinations are most valuable in the diagnostic assessment of patients with established or suspected allergic desease. In normal subjects, IgE values are related to age, with normal values peaking around 10 – 14 years. Infants and children with family history of atopic allergy are at increased risk of developing disease and constitute a prime population for screening. Studies have shown that conditions such as asthma, rhinitis, eczema, urticaria, dermatitis and some parasitic infections lead to increased IgE levels. Asthma, hay fever and atopic eczema patients may produce levels 3 – 10 times those of normal patients.

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