Lupin ELISA Kit (ARG80804)

$799.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity
Tested Application ELISA
Specificity
Target Name Lupin
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 0.2 ppm
Detection Range
Sample Type food (extraction, dilution)
Sample Volume 100 ul
Precision

CV: <10%

Full Name Lupin
Alternate Names Check alternative names for the Lupin ELISA Kit

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Application Instructions

 

Assay Time 20, 20 min (RT), 20 min (RT/dark)


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Lupin ELISA Kit  Download
size

96 wells

Cat No.

ARG80804

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

0.2 ppm

Sample type

food (extraction, dilution)

Sample Volume

100 ul

Precision

CV: <10%

Target name

Lupin

Assay time

20, 20 min (RT), 20 min (RT/dark)

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

Lupin belongs to the legume family. With about 35-45% the fraction of proteins in lupin seed is very high. Some of these proteins, predominantly those in the alpha, beta and gamma-Conglutin fractions are known for being allergenic, due to cross-reaction to allergens of soy and peanut. Because of its convenient nutritional properties the use of lupin flour as a food ingredient is highly increasing. Lupin is often used in dietary products, especially for milk allergic people, vegetarians and patients with celiac disease. In food production three (sweet) lupin species are commonly used: Lupinus albus, Lupinus angustifolius and Lupinus luteus. For lupin allergic persons hidden lupin allergens in food are a critical problem. Already very low amounts of lupin can cause allergic reactions, which may lead to anaphylactic shock in severe cases. Because of this, lupin allergic per?sons must strictly avoid the consumption of lupin containing food. Cross-contamination, most?ly in consequence of the production pro?cess, is often noticed. This explains why in many cases the existence of lupin residues in food cannot be excluded. For this reason sensitive detection systems for lupin residues in food?stuffs are required. The Lupin ELISA represents a highly sensitive detection system for lupin and is particularly capable of the quantification of residues in sausage, bakery products, potato products, ketchup and juices. Due to high cross-reactivity the test is suitable for the detection of all commonly used lupin species.

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