Mouse IL12 p70 ELISA Kit (ARG80201)

$499.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Mouse
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Mouse IL-12 p35, IL-12/IL-23 p40 monomer, IL-12/IL-23 p40 dimer, IL-23;human IL-12 p70, IL-12 p35, IL-12/IL-23 p40 monomer;canine IL-12 p70;feline IL-12 p70
Target Name IL12 p70
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 8 pg/ml
Detection Range 15.6-1000 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Sample Volume 100 ul
Precision

CV: <10%

Full Name interleukin 12a
Alternate Names Check alternative names for the Mouse IL12 p70 ELISA Kit

Expand

IL12A Elisa kits| IL12B Elisa kits|CLMF p35 Elisa kits|CLMF p40 Elisa kits|Cytotoxic lymphocyte maturation factor 35 kDa subunit Elisa kits|Cytotoxic lymphocyte maturation factor 40 kDa subunit Elisa kits|Interleukin 12 alpha chain Elisa kits|Interleukin-12 beta chain Elisa kits|NK cell stimulatory factor chain 1 Elisa kits|NK cell stimulatory factor chain 2 Elisa kits|NKSF1 Elisa kits|NKSF2 Elisa kits


Application Instructions

 

Assay Time 4 hours


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Mouse IL12 p70 ELISA Kit  Download
size

96 wells

Cat No.

ARG80201

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

8 pg/ml

Detection range

15.6-1000 pg/ml

Sample type

Serum, Plasma, Cell culture supernatants

Sample Volume

100 ul

Precision

CV: <10%

Target name

IL12 p70

Assay time

4 hours

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

Interleukin-12 is a disulfide-linked heterodimeric protein (p70), composed of two subunits, p35 and p40, which are encoded by two different genes. Accumulating data indicate that p40 secretion precedes that of IL-12 expression. In addition, to its ability to covalently bind to p35 to form IL-12, p40 can bind to p19 to form IL-23. Additionally, p40 can associate itself through disulfide linkage to form a dimer that acts as a potent IL12 antagonist. Recombinant murine IL-12 p40 is a 40.0 kDa protein containing 313 amino acid residues.

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