Mouse IL4 ELISA Kit (ARG80198)

$499.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Mouse
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human IL-4, IL-4R
Target Name IL4
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 4 pg/ml
Detection Range 7.8 – 500 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Sample Volume 100 ul
Precision

CV: <10%

Full Name interleukin 4
Alternate Names Check alternative names for the Mouse IL4 ELISA Kit

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Application Instructions

 

Assay Time 4 hours


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Mouse IL4 ELISA Kit  Download
size

96 wells

Cat No.

ARG80198

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

4 pg/ml

Detection range

7.8 – 500 pg/ml

Sample type

Serum, Plasma, Cell culture supernatants

Sample Volume

100 ul

Precision

CV: <10%

Target name

IL4

Assay time

4 hours

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

IL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naive CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

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