Mouse IL5 ELISA kit (ARG80907)

$499.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Mouse
Tested Application ELISA
Specificity No significant cross-reactivity or interference with GM-CSF, IL3, IL5Ralpha.
Target Name Mouse IL5
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 8 pg/ml
Detection Range
Sample Type Serum, Plasma, Cell culture supernatants
Sample Volume 100 ul
Precision

CV: <10%

Full Name interleukin 5
Alternate Names Check alternative names for the Mouse IL5 ELISA kit

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Application Instructions

 

Assay Time 4 hours


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Mouse IL5 ELISA kit  Download
size

96 wells

Cat No.

ARG80907

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

8 pg/ml

Sample type

Serum, Plasma, Cell culture supernatants

Sample Volume

100 ul

Precision

CV: <10%

Target name

Mouse IL5

Assay time

4 hours

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

The IL-5 gene is located on chromosome 11 in the mouse, and chromosome 5 in humans, in close proximity to the genes encoding IL-3, IL-4, and granulocyte-macrophage colony-stimulating factor (GM-CSF), which are often co-expressed in TH2 cells. Interleukin-5 is also expressed by eosinophils[4] and has been observed in the mast cells of asthmatic airways by immunohistochemistry. IL-5 expression is regulated by several transcription factors including GATA3. [IL-5 wiki] IL5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL5 plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of IL5 may be related to pathogenesis of eosinophil-dependent inflammatory diseases. IL5 functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). [provided by RefSeq, Jul 2013]

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