Mouse Monoclonal ABCB5 antibody [11A2] (STJ96976)

$99.00$319.00

Reactivity: Human
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal ABCB5 antibody [11A2] (STJ96976)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB

Recommended dilution: WB 1:2000

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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ABCB5 antibody,|ABCB 5 antibody|ABCB5 antibody|ABCB5 P-gp antibody|ABCB5_HUMAN antibody|ABCB5alpha antibody|ABCB5beta antibody|ATP binding cassette protein antibody|ATP binding cassette sub family B (MDR/TAP) member 5 antibody|ATP binding cassette sub family B member 5 antibody|ATP-binding cassette sub-family B member 5 antibody|EST422562 antibody|P glycoprotein ABCB5 antibody|P-glycoprotein ABCB5 antibody|Anti-ABCB5 antibody (ab77549)
SCBT cat No: sc-517210|sc-104019|

Name

ABCB5 Monoclonal Antibody

Catalogue No.

STJ96976

Reactivity

Human

Specificity

The antibody detects endogenous ABCB5 proteins.

Immunogen

Synthetic Peptide

Host

Mouse

Applications

WB

Recommended dilution

WB 1:2000

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

ABCB5 Antibody was tube-contained. PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Purification

ABCB5 Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

ATP-binding cassette sub-family B member 5 antibody, ABCB5 P-gp antibody, P-glycoprotein ABCB5 antibody

Database links

Human UniProt/Swiss-Prot:Q2M3G0;Mouse UniPort/Swiss-Prot: B5X0E4;Rat UniProt/Swiss-Port: D4AEL4;Human Entrez Gene: 340273;Mouse Entrez Gene: 77706;

Protein names

ATP-binding cassette sub-family B member 5 , ABCB5 P-gp , P-glycoprotein ABCB5

Protein function

Drug efflux transporter present in a number of stem cells that acts as a regulator of cellular differentiation. Able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Specifically present in limbal stem cells, where it plays a key role in corneal development and repair.

Protein tissue specificity

Expressed by CD133-expressing progenitor cells among epidermal melanocytes (at protein level). Widely expressed with specific expression in pigment cells. Highly expressed in several malignant tissues: highly expressed in clinical melanomas, with low expression in normal skin. In melanoma, marks malignant melanoma-initiating cells (MMIC), in which clinical virulence resides as a consequence of unlimited self-renewal capacity, resulting in inexorable tumor progression and metastasis. Also highly expressed in a number of leukemia cells. Expressed in basal limbal epithelium.

Protein sequence and domain

Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. [View classification] / Contains 2 ABC transmembrane type-1 domains. / Contains 2 ABC transporter domains.

Protein cellular localization

Cell membrane / Multi-pass membrane protein

Research area

All research areas>Transport and Trafficking>ABCB5
(View all antibody categories related to Transport and Trafficking)

Note

AntibodyPlus can customize ABCB5 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human liver cancer tissue

1: ABCB5 Monoclonal Antibody(11A2)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human liver cancer tissue

1: ABCB5 Monoclonal Antibody(11A2)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human liver cancer tissue

1: ABCB5 Monoclonal Antibody(11A2)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human lung tissue

1: ABCB5 Monoclonal Antibody(11A2)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human lung tissue

1: ABCB5 Monoclonal Antibody(11A2)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human stomach cancer tissue

1: ABCB5 Monoclonal Antibody(11A2)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human stomach cancer tissue

1: ABCB5 Monoclonal Antibody(11A2)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human breast cancer tissue

1: ABCB5 Monoclonal Antibody(11A2) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human liver tissue

1: ABCB5 Monoclonal Antibody(11A2) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human liver cancer tissue

1: ABCB5 Monoclonal Antibody(11A2) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human lung cancer tissue

1: ABCB5 Monoclonal Antibody(11A2) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human kidney tissue

1: ABCB5 Monoclonal Antibody(11A2) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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