Mouse Monoclonal Actin beta antibody (KLH) [8H10D10] (STJ97814)

$379.00

Reactivity: Human, Mouse, Rat, Hamster, Monkey
Applications: WB, IF, FC, ELISA
Conjugation: KLH
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal Actin beta antibody (KLH) [8H10D10] (STJ97814)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IF, FC, ELISA

Recommended dilution: WB 1:500-1:2000; IF 1:200-1:1000; FC 1:200-1:400; ELISA 1:10000

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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ACTB antibody,|A26C1A antibody|A26C1B antibody|ACTB antibody|ACTB_HUMAN antibody|Actin cytoplasmic 1 antibody|Actin, cytoplasmic 1, N-terminally processed antibody|Actx antibody|b actin antibody|Beta cytoskeletal actin antibody|Beta-actin antibody|BRWS1 antibody|E430023M04Rik antibody|MGC128179 antibody|PS1TP5 binding protein 1 antibody|PS1TP5BP1 antibody|Anti-beta Actin antibody [mAbcam 8224] – Loading Control (ab8224)
SCBT cat No: sc-56499|sc-166524|sc-7454|sc-17829|sc-15335|sc-7453|sc-135819|sc-393495|

 

Name

Actin beta Monoclonal Antibody

Catalogue No.

STJ97814

Reactivity

Human, Mouse, Rat, Hamster, Monkey

Specificity

Actin beta Monoclonal Antibody detects endogenous levels of Actin beta protein.

Immunogen

Synthetic peptide corresponding to amino-terminal residues of human Actin beta, conjugated to KLH

Host

Mouse

Applications

WB, IF, FC, ELISA

Recommended dilution

WB 1:500-1:2000; IF 1:200-1:1000; FC 1:200-1:400; ELISA 1:10000

Clonality

Monoclonal

Conjugation

KLH

Isotype

IgG2b

Formulation

Actin beta Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.

Concentration

1 mg/ml

Purification

Actin beta Antibody was purified using affinity purification.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Actin, cytoplasmic 1 antibody, Beta-actin antibody

Database links

Human UniProt/Swiss-Prot:P60709;Mouse UniPort/Swiss-Prot: P60710;Rat UniProt/Swiss-Port: P60711;Human Entrez Gene: 60;Mouse Entrez Gene: 11461;Rat Entrez Gene: Rn.94978

Protein names

Actin, cytoplasmic 1 , Beta-actin

Protein function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Involvement in disease

Dystonia, juvenile-onset (DJO) [MIM:607371]: A form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. Patients with juvenile-onset dystonia manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Baraitser-Winter syndrome 1 (BRWS1) [MIM:243310]: A rare developmental disorder characterized by the combination of congenital ptosis, high-arched eyebrows, hypertelorism, ocular colobomata, and a brain malformation consisting of anterior-predominant lissencephaly. Other typical features include postnatal short stature and microcephaly, intellectual disability, seizures, and hearing loss. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

Belongs to the actin family.

Protein post-translational modifications

ISGylated. / Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity). / Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. / (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding . The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners .

Protein cellular localization

Cytoplasm > cytoskeleton

Research area

All research areas>Structural Proteins>Actin
(View all antibody categories related to Structural Proteins)

Note

AntibodyPlus can customize Actin beta Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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