Mouse Monoclonal AFP alpha 1 Fetoprotein antibody [17C5] (STJ96977)

$99.00$319.00

Reactivity: Human
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal AFP alpha 1 Fetoprotein antibody [17C5] (STJ96977)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB

Recommended dilution: WB 1:2000

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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AFP antibody, HPAFP antibody,|Afp antibody|Alpha fetoglobulin antibody|Alpha fetoprotein antibody|Alpha fetoprotein precursor antibody|Alpha-1-fetoprotein antibody|Alpha-fetoglobulin antibody|Alpha-fetoprotein antibody|alpha-fetoprotein, Hereditary persistence of, included antibody|FETA antibody|FETA_HUMAN antibody|Hereditary persistence of alpha fetoprotein antibody|HPAFP antibody|Anti-alpha 1 Fetoprotein antibody [AFP-01] (ab3980)
SCBT cat No: sc-80464|sc-130302|sc-69857|sc-51506|sc-8108|sc-8399|sc-166450|sc-166325|sc-15375|sc-166335|

Name

AFP alpha 1 Fetoprotein Monoclonal Antibody

Catalogue No.

STJ96977

Reactivity

Human

Specificity

The antibody detects endogenous AFP proteins.

Immunogen

Synthetic Peptide

Host

Mouse

Applications

WB

Recommended dilution

WB 1:2000

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

AFP alpha 1 Fetoprotein Antibody was tube-contained. PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Purification

AFP alpha 1 Fetoprotein Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Alpha-fetoprotein antibody, Alpha-1-fetoprotein antibody, Alpha-fetoglobulin antibody

Database links

Human UniProt/Swiss-Prot:P02771;Mouse UniPort/Swiss-Prot: P02772;Rat UniProt/Swiss-Port: G3V6D0;Human Entrez Gene: 174;Mouse Entrez Gene: 11576;Rat Entrez Gene: Rn.9174

Protein names

Alpha-fetoprotein , Alpha-1-fetoprotein , Alpha-fetoglobulin

Protein function

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

Protein tissue specificity

Plasma. Synthesized by the fetal liver and yolk sac.

Involvement in disease

Alpha-fetoprotein deficiency (AFPD) [MIM:615969]: A benign condition characterized by undetectable AFP levels in the amniotic fluid. Affected individuals are asymptomatic and present normal development. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Alpha-fetoprotein, hereditary persistence (HPAFP) [MIM:615970]: A benign autosomal dominant condition characterized by continued expression of alpha-fetoprotein in adult life. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

Belongs to the ALB/AFP/VDB family. / Contains 3 albumin domains.

Protein post-translational modifications

Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence. / Sulfated.

Protein cellular localization

Secreted

Research area

All research areas>Structural Proteins>AFP
(View all antibody categories related to Structural Proteins)

Note

AntibodyPlus can customize AFP alpha 1 Fetoprotein Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human breast cancer tissue

1: AFP alpha 1 Fetoprotein Monoclonal Antibody(17C5)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human breast cancer tissue

1: AFP alpha 1 Fetoprotein Monoclonal Antibody(17C5)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human breast cancer tissue

1: AFP alpha 1 Fetoprotein Monoclonal Antibody(17C5)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human liver cancer tissue

1: AFP alpha 1 Fetoprotein Monoclonal Antibody(17C5)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human liver cancer tissue

1: AFP alpha 1 Fetoprotein Monoclonal Antibody(17C5)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human liver cancer tissue

1: AFP alpha 1 Fetoprotein Monoclonal Antibody(17C5)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human stomach cancer tissue

1: AFP alpha 1 Fetoprotein Monoclonal Antibody(17C5)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human stomach cancer tissue

1: AFP alpha 1 Fetoprotein Monoclonal Antibody(17C5)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human breast cancer tissue

1: AFP alpha 1 Fetoprotein Monoclonal Antibody(17C5) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human liver tissue

1: AFP alpha 1 Fetoprotein Monoclonal Antibody(17C5) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human liver cancer tissue

1: AFP alpha 1 Fetoprotein Monoclonal Antibody(17C5) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human lung cancer tissue

1: AFP alpha 1 Fetoprotein Monoclonal Antibody(17C5) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human kidney tissue

1: AFP alpha 1 Fetoprotein Monoclonal Antibody(17C5) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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