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Mouse Monoclonal AIF-M1 antibody [4E7] (STJ97819)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, IF, FC, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; FC 1:200-1:400; ELISA 1:10000
Recommended protocols: check protocols
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Check alternative names for the antibodyExpand
AIFM1 antibody, AIF antibody, PDCD8 antibody,|AIFM1 antibody|AIFM1_HUMAN antibody|Apoptosis inducing factor 1, mitochondrial antibody|Apoptosis inducing factor antibody|Apoptosis inducing factor, mitochondrion associated, 1 antibody|Apoptosis-inducing factor 1 antibody|CMTX4 antibody|COWCK antibody|COXPD6 antibody|Harlequin antibody|Hq antibody|mAIF antibody|MGC111425 antibody|MGC5706 antibody|mitochondrial antibody|Neuropathy, axonal motor-sensory, with deafness and mental retardation antibody|neuropathy, axonal, motor-sensory with deafness and mental retardation (Cowchock syndrome) antibody|PDCD 8 antibody|PDCD8 antibody|Programmed cell death 8 (apoptosis inducing factor) antibody|Programmed cell death 8 antibody|Programmed cell death 8 isoform 1 antibody|Programmed cell death 8 isoform 2 antibody|Programmed cell death 8 isoform 3 antibody|Programmed cell death protein 8 antibody|Programmed cell death protein 8 mitochondrial antibody|Programmed cell death protein 8 mitochondrial precursor antibody|Programmed cell death protein 8 mitochondrial precursor antibody|Striatal apoptosis inducing factor antibody|Anti-AIF antibody [E20] – Mitochondrial Marker (ab32516)
SCBT cat No: sc-55519|sc-9416|sc-13116|sc-390619|sc-5586|sc-9417|
AIF-M1 Monoclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat, Monkey
AIF-M1 Monoclonal Antibody detects endogenous levels of AIF-M1 protein.
Purified recombinant fragment of human AIF-M1 expressed in E Coli
WB, IHC, IF, FC, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; FC 1:200-1:400; ELISA 1:10000
AIF-M1 Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.
AIF-M1 Antibody was purified using affinity purification.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Apoptosis-inducing factor 1, mitochondrial antibody, Programmed cell death protein 8 antibody
|Protein names|| |
Apoptosis-inducing factor 1, mitochondrial , Programmed cell death protein 8
|Protein function|| |
Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces ‘parthanatos’ i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. / FAD / 1.53 mM for NADH / 26 µM for cytochrome c
|Protein tissue specificity|| |
Detected in muscle and skin fibroblasts (at protein level). Isoform 5 is frequently down-regulated in human cancers.
|Involvement in disease|| |
Combined oxidative phosphorylation deficiency 6 (COXPD6) [MIM:300816]: A mitochondrial disease resulting in a neurodegenerative disorder characterized by psychomotor delay, hypotonia, areflexia, muscle weakness and wasting. Some patients manifest prenatal ventriculomegaly and severe postnatal encephalomyopathy. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Cowchock syndrome (COWCK) [MIM:310490]: An X-linked recessive neuromuscular disorder characterized by early childhood onset of a slowly progressive axonal sensorimotor neuropathy associated in some patients with sensorineural deafness and cognitive impairment. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Deafness, X-linked, 5 (DFNX5) [MIM:300614]: A form of hearing loss characterized by absent or severely abnormal auditory brainstem response, abnormal middle ear reflexes, abnormal speech discrimination, loss of outer hair cell function, and cochlear nerve hypoplasia. DFNX5 patients manifest auditory neuropathy with childhood onset, associated with distal sensory impairment affecting the peripheral nervous system. . Note: The disease is caused by mutations affecting the gene represented in this entry.
|Protein sequence and domain|| |
Belongs to the FAD-dependent oxidoreductase family.
|Protein post-translational modifications|| |
Under normal conditions, a 54-residue N-terminal segment is first proteolytically removed during or just after translocation into the mitochondrial intermembrane space (IMS) by the mitochondrial processing peptidase (MPP) to form the inner-membrane-anchored mature form (AIFmit). During apoptosis, it is further proteolytically processed at amino-acid position 101 leading to the generation of the mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis in a caspase-independent manner. / Ubiquitination by XIAP/BIRC4 does not lead to proteasomal degradation. Ubiquitination at Lys-255 by XIAP/BIRC4 blocks its ability to bind DNA and induce chromatin degradation, thereby inhibiting its ability to induce cell death.
|Protein cellular localization|| |
Mitochondrion intermembrane space / Mitochondrion inner membrane / Cytoplasm / Nucleus / Cytoplasm > perinuclear region / Mitochondrion intermembrane space / Mitochondrion inner membrane / Cytoplasm
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St John’s Laboratory Ltd.
|Product type|| |
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