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Mouse Monoclonal Akt3 antibody [3B12F3F10; 2D2B11B11; 2D2B11H5] (STJ97822)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-1:2000; ELISA 1:10000
Recommended protocols: check protocols
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Check alternative names for the antibodyExpand
AKT3 antibody, PKBG antibody,|AKT antibody|AKT1 antibody|AKT1 kinase antibody|AKT1m antibody|AKT2 antibody|AKT2 kinase antibody|Akt3 antibody|AKT3_HUMAN antibody|CAKT antibody|CWS6 antibody|DKFZp434N0250 antibody|HIHGHH antibody|kinase Akt1 antibody|MGC99656 antibody|MPPH antibody|Murine thymoma viral (v-akt) homolog 2 antibody|PKB ALPHA antibody|PKB antibody|PKB beta antibody|PKB gamma antibody|PKB-GAMMA antibody|PKB/Akt antibody|PKBALPHA antibody|PKBB antibody|PKBBETA antibody|PKBG antibody|PKBGAMMA antibody|PRKBA antibody|PRKBB antibody|PRKBG antibody|Protein kinase Akt 2 antibody|Protein kinase Akt-3 antibody|Protein kinase B alpha antibody|Protein kinase B antibody|Protein kinase B beta antibody|Protein kinase B gamma antibody|Proto oncogene c Akt antibody|RAC ALPHA antibody|RAC alpha serine/threonine protein kinase antibody|RAC antibody|RAC BETA antibody|RAC beta serine/threonine protein kinase antibody|RAC PK alpha antibody|RAC PK beta antibody|rac protein kinase alpha antibody|rac protein kinase beta antibody|RAC-gamma antibody|RAC-gamma serine/threonine-protein kinase antibody|RAC-PK-gamma antibody|RACALPHA antibody|RACalpha serine/threonine kinase antibody|RACBETA antibody|RACgamma antibody|RACgamma serine/threonine protein kinase antibody|RACPKgamma antibody|serine threonine protein kinase antibody|STK-2 antibody|STK2 antibody|thymoma viral proto oncogene 1 antibody|thymoma viral proto oncogene antibody|V akt murine thymoma viral oncogene homolog 1 antibody|V akt murine thymoma viral oncogene homolog 2 antibody|V akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) antibody|V akt murine thymoma viral oncogene homolog 3 antibody|vakt murine thymoma viral oncogene homolog 1 antibody|vakt murine thymoma viral oncogene homolog 2 antibody|vakt murine thymoma viral oncogene homolog 3 antibody|Anti-AKT1/2/3 antibody [EPR16798] (ab179463)
SCBT cat No: sc-11520|sc-134254|sc-11521|
Akt3 Monoclonal Antibody
|Catalogue No.|| |
Akt3 Monoclonal Antibody detects endogenous levels of Akt3 protein.
Purified recombinant fragment of Akt3 expressed in E Coli
|Recommended dilution|| |
WB 1:500-1:2000; ELISA 1:10000
Akt3 Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.
Akt3 Antibody was purified using affinity purification.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
RAC-gamma serine/threonine-protein kinase antibody, Protein kinase Akt-3 antibody, Protein kinase B gamma antibody, PKB gamma antibody, RAC-PK-gamma antibody, STK-2 antibody
|Protein names|| |
RAC-gamma serine/threonine-protein kinase , Protein kinase Akt-3 , Protein kinase B gamma , PKB gamma , RAC-PK-gamma , STK-2
|Protein function|| |
AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis. / ATP + a protein = ADP + a phosphoprotein. / Two specific sites, one in the kinase domain (Thr-305) and the other in the C-terminal regulatory region (Ser-472), need to be phosphorylated for its full activation (By similarity). IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. / 87.9 µM for ATP (for purified and in vitro activated AKT3) / 12.4 µM for peptide substrate (for purified and in vitro activated AKT3) / 118.7 µM for ATP (for recombinant myristoylated AKT3 expressed and immunoprecipitated from Rat-1 cells) / 2.3 µM for peptide substrate (for recombinant myristoylated AKT3 expressed and immunoprecipitated from Rat-1 cells)
|Protein tissue specificity|| |
In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
|Involvement in disease|| |
Note: AKT3 is a key modulator of several tumors like melanoma, glioma and ovarian cancer. Active AKT3 increases progressively during melanoma tumor progression with highest levels present in advanced-stage metastatic melanomas. Promotes melanoma tumorigenesis by decreasing apoptosis. Plays a key role in the genesis of ovarian cancers through modulation of G2/M phase transition. With AKT2, plays a pivotal role in the biology of glioblastoma.; Megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 2 (MPPH2) [MIM:615937]: A syndrome characterized by megalencephaly, hydrocephalus, and polymicrogyria; polydactyly may also be seen. There is considerable phenotypic similarity between this disorder and the megalencephaly-capillary malformation syndrome. . Note: The disease is caused by mutations affecting the gene represented in this entry.
|Protein sequence and domain|| |
Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI3K) results in its targeting to the plasma membrane. / Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. / Contains 1 AGC-kinase C-terminal domain. / Contains 1 PH domain. / Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
Phosphorylation on Thr-305 and Ser-472 is required for full activity. / Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes ‘Lys-48’-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. / O-GlcNAcylation at Thr-302 and Thr-309 inhibits activating phosphorylation at Thr-305 via disrupting the interaction between AKT and PDK1.
|Protein cellular localization|| |
Nucleus / Cytoplasm / Membrane / Peripheral membrane protein
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St John’s Laboratory Ltd.
|Product type|| |
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