Mouse Monoclonal alpha-tubulin (Acetyl Lys40) antibody [4A8] (STJ97537)

$99.00$319.00

Reactivity: Human, Rat, Mouse
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal alpha-tubulin (Acetyl Lys40) antibody [4A8] (STJ97537)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC

Recommended dilution: WB 1:1000-2000; IHC 1:50-100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for alpha-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8).

Alternative names:

Check alternative names for the antibody

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TUBA1B antibody,|alpha 1b antibody|Alpha-tubulin ubiquitous antibody|K-ALPHA-1 antibody|Tubulin alpha-1B chain antibody|Tubulin alpha-ubiquitous chain antibody|Tubulin K-alpha-1 antibody|Anti-Acetylated alpha Tubulin antibody [6-11B-1] (ab24610)
SCBT cat No: sc-134238|

Name

alpha-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8)

Catalogue No.

STJ97537

Reactivity

Human, Rat, Mouse

Specificity

alpha-tubulin (Acetyl Lys40) Mouse Monoclonal Antibody(4A8) detects endogenous levels of alpha-tubulin (Acetyl Lys40)

Immunogen

Synthetic Peptide

Host

Mouse

Applications

WB, IHC

Recommended dilution

WB 1:1000-2000; IHC 1:50-100

Clonality

Monoclonal

Conjugation

Unconjugated

Formulation

alpha-tubulin (Acetyl Lys40) Antibody(4A8) was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

alpha-tubulin (Acetyl Lys40) Antibody(4A8) was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Tubulin alpha-1B chain antibody, Alpha-tubulin ubiquitous antibody, Tubulin K-alpha-1 antibody, Tubulin alpha-ubiquitous chain antibody

Database links

Human UniProt/Swiss-Prot:P68363;Mouse UniPort/Swiss-Prot: P05213;Rat UniProt/Swiss-Port: Q6P9V9;Human Entrez Gene: 7277;Mouse Entrez Gene: 22143;Rat Entrez Gene: Rn.234326

Protein names

Tubulin alpha-1B chain , Alpha-tubulin ubiquitous , Tubulin K-alpha-1 , Tubulin alpha-ubiquitous chain

Protein function

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Protein sequence and domain

Belongs to the tubulin family.

Protein post-translational modifications

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group . Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold . / Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable). / Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling (By similarity). / Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.

Protein cellular localization

Cytoplasm > cytoskeleton

Research area

All research areas>Structural Proteins>Tubulin
(View all antibody categories related to Structural Proteins)

Note

AntibodyPlus can customize alpha-tubulin (Acetyl Lys40) Antibody(4A8) according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human liver cancer tissue

1: alpha-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human liver cancer tissue

1: alpha-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse lung tissue

1: alpha-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse lung tissue

1: alpha-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat spinal cord tissue

1: alpha-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat spinal cord tissue

1: alpha-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human uterus tissue

1: alpha-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human liver tissue

1: alpha-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human liver cancer tissue

1: alpha-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue

1: alpha-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat heart tissue

1: alpha-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat kidney tissue

1: alpha-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse heart tissue

1: alpha-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse kidney tissue

1: alpha-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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