Mouse Monoclonal Anti-4E-BP2 antibody (STJ99276)

$99.00$319.00

Reactivity: Transfected
Applications: WB, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal Anti-4E-BP2 antibody (STJ99276)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-2000; ELISA 1:10000-20000

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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|4EBP1 antibody|4EBP2 antibody|4EBP3 antibody|eIF4E binding protein 1 antibody|eIF4E binding protein 2 antibody|eIF4E binding protein 3 antibody|Eukaryotic translation initiation factor 4E binding protein 1 antibody|Eukaryotic translation initiation factor 4E binding protein 2 antibody|Eukaryotic translation initiation factor 4E binding protein 3 antibody|PHAS1 antibody|Phospho anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (T45) antibody [EPR2169Y] (ab68187)
SCBT cat No: sc-134232|

Name

Anti-4E-BP2 antibody

Catalogue No.

STJ99276

Reactivity

Transfected

Immunogen

Recombinant protein of human 4E-BP2.

Host

Mouse

Applications

WB, ELISA

Recommended dilution

WB 1:500-2000; ELISA 1:10000-20000

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Molecular weight

15-20kDa

Formulation

Anti-4E-BP2 antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Anti-4E-BP2 antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

4EBP1 antibody, 4EBP2 antibody, 4EBP3 antibody, eIF4E binding protein 1 antibody, eIF4E binding protein 2 antibody, eIF4E binding protein 3 antibody, Eukaryotic translation initiation factor 4E binding protein 1 antibody, Eukaryotic translation initiation factor 4E binding protein 2 antibody, Eukaryotic translation initiation factor 4E binding protein 3 antibody, PHAS1 antibody

Database links

UniProt/Swiss-Prot:Q13542

Protein function

Repressor of translation initiation involved in synaptic plasticity, learning and memory formation (By similarity). Regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form of EIF4EBP2 competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation (PubMed:25533957). EIF4EBP2 is enriched in brain and acts as a regulator of synapse activity and neuronal stem cell renewal via its ability to repress translation initiation (By similarity). Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways (By similarity). .

Protein sequence and domain

Belongs to the eIF4E-binding protein family. The TOS motif mediates interaction with RPTOR, leading to promote phosphorylation by mTORC1 complex. ; Intrinsically disordered protein that undergoes folding upon phosphorylation (PubMed:25533957). Hypophosphorylated form interacts strongly with EIF4E using (1) the YXXXXLPhi motif, that undergoes a disorder-to-helix transition upon binding and (2) the secondary EIF4E binding sites (residues 78-82) (PubMed:24207126, PubMed:25533957). Phosphorylation at Thr-37 and Thr-46 induces folding of region encompassing residues from Pro-18 to Arg-62 of into a four-stranded beta-domain that sequesters the helical YXXXXLPhi motif into a buried beta-strand, blocking accessibility to EIF4E. Protein phosphorylated at Thr-37 and Thr-46 is however unstable and subsequent phosphorylation at Ser-65, Thr-70 and Ser-83 is required to stabilize the fold, decreasing affinity for EIF4E by a factor of 4000 (PubMed:24207126, PubMed:25533957).

Protein post-translational modifications

Phosphorylation at Thr-37, Thr-46, Ser-65, Thr-70 and Ser-83 is mediated by MTOR and corresponds to the hyperphosphorylated form: it abolishes binding to EIF4E by inducing folding of intrinsically disordered regions (PubMed:24207126, PubMed:25533957). First phosphorylated at Thr-37 and Thr-46 by MTOR, inducing folding of region encompassing residues from Pro-18 to Arg-62 of into a four-stranded beta-domain that sequesters the helical YXXXXLPhi motif into a partly buried beta-strand, blocking accessibility to EIF4E. Protein phosphorylated at Thr-37 and Thr-46 is however unstable and subsequent phosphorylation at Ser-65, Thr-70 and Ser-83 is required to stabilize the fold, decreasing affinity for EIF4E by a factor of 4000 (PubMed:24207126, PubMed:25533957). Phosphorylated in response to insulin, EGF and PDGF. .; Deamidated at Asn-99 and Asn-102 to aspartate (Asp) in brain. Deamidation promotes interaction with RPTOR, subsequent phosphorylation by mTORC1 and increased translation, leading to impair kinetics of excitatory synaptic transmission. Deamidation takes place during postnatal development, when the PI3K-Akt-mTOR signaling is reduced, suggesting it acts as a compensatory mechanism to promote translation despite attenuated PI3K-Akt-mTOR signaling in neuron development. Deamidation converts Asn residues into a mixture of Asp and isoaspartate; interactions with PCMT1 is required to prevent isoaspartate accumulation and convert isoaspartate to Asp. .

Research area

All research areas>Signaling Intermediates>4E-BP
(View all antibody categories related to Signaling Intermediates)

Note

AntibodyPlus can customize Anti-4E-BP2 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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