Mouse Monoclonal Anti-ATG7 antibody (STJ98914)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal Anti-ATG7 antibody (STJ98914)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-2000; ELISA 1:10000-20000

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Anti-ATG7 antibody (STJ98914).

Alternative names:

Check alternative names for the antibody

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|1810013K23Rik antibody|DKFZp434N0735 antibody|GSA 7 antibody|GSA7 antibody|hAGP7 antibody|Ubiquitin activating enzyme E1 like protein antibody|Ubiquitin-activating enzyme E1-like protein antibody|Ubiquitin-like modifier-activating enzyme ATG7 antibody|Anti-Apg7 antibody [EP1759Y] (ab52472)
SCBT cat No: sc-376212|sc-33211|sc-12497|sc-8668|

Name

Anti-ATG7 antibody

Catalogue No.

STJ98914

Reactivity

Human, Mouse, Rat

Specificity

Anti-ATG7 antibody detects endogenous ATG7 protein.

Immunogen

Recombinant Protein of ATG7.

Host

Mouse

Applications

WB, ELISA

Recommended dilution

WB 1:500-2000; ELISA 1:10000-20000

Clonality

Monoclonal

Conjugation

Unconjugated

Molecular weight

78kDa

Formulation

Anti-ATG7 antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Anti-ATG7 antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

1810013K23Rik antibody, DKFZp434N0735 antibody, GSA 7 antibody, GSA7 antibody, hAGP7 antibody, Ubiquitin activating enzyme E1 like protein antibody, Ubiquitin-activating enzyme E1-like protein antibody, Ubiquitin-like modifier-activating enzyme ATG7 antibody

Database links

UniProt/Swiss-Prot:O95352

Protein function

E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. .

Protein tissue specificity

Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. .

Protein sequence and domain

Belongs to the ATG7 family. The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12. ; The N-terminal FAP motif (residues 15 to 17) is essential for the formation of the ATG89-PE and ATG5-ATG12 conjugates.

Protein post-translational modifications

Acetylated by EP300. .

Protein cellular localization

Cytoplasm . Preautophagosomal structure . Note=Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme. .

Note

AntibodyPlus can customize Anti-ATG7 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Mouse spleen tissue

1: ATG7 Mouse Monoclonal Antibody(3D6)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse spleen tissue

1: ATG7 Mouse Monoclonal Antibody(3D6)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human uterus tissue

1: ATG7 Mouse Monoclonal Antibody(3D6) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat lung tissue

1: ATG7 Mouse Monoclonal Antibody(3D6) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat kidney tissue

1: ATG7 Mouse Monoclonal Antibody(3D6) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat spleen tissue

1: ATG7 Mouse Monoclonal Antibody(3D6) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse lung tissue

1: ATG7 Mouse Monoclonal Antibody(3D6) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse kidney tissue
1: ATG7 Mouse Monoclonal Antibody(3D6) was diluted at 1:200 (4 degree Celsius

1: ATG7 Mouse Monoclonal Antibody(3D6) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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