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Mouse Monoclonal Anti-Bcl-2 antibody (STJ99291)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-2000; ELISA 1:10000-20000
Recommended protocols: check protocols
Click or hover above images to see image description for Anti-Bcl-2 antibody.
Check alternative names for the antibodyExpand
|Apoptosis regulator Bcl 2 antibody|Apoptosis regulator Bcl-2 antibody|Apoptosis regulator Bcl2 antibody|AW986256 antibody|B cell CLL/lymphoma 2 antibody|B cell leukemia/lymphoma 2 antibody|Bcl-2 antibody|Bcl2 antibody|BCL2_HUMAN antibody|C430015F12Rik antibody|D630044D05Rik antibody|D830018M01Rik antibody|Leukemia/lymphoma, B-cell, 2 antibody|Oncogene B-cell leukemia 2 antibody|PPP1R50 antibody|Protein phosphatase 1, regulatory subunit 50 antibody|Anti-Bcl-2 antibody [E17] (ab32124)
SCBT cat No: sc-509|sc-56015|sc-23960|sc-130307|sc-578|sc-70411|sc-130308|sc-65392|sc-783|sc-7382|sc-492|sc-56018|sc-81002|sc-169990|sc-169989|sc-32741|sc-185|sc-13038|sc-70414|
|Catalogue No.|| |
Anti-Bcl-2 antibody detects endogenous levels of BCL2.
Recombinant protein corresponding to fragment (1-195aa) BCL2.
|Recommended dilution|| |
WB 1:500-2000; ELISA 1:10000-20000
|Molecular weight|| |
Anti-Bcl-2 antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Anti-Bcl-2 antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Apoptosis regulator Bcl 2 antibody, Apoptosis regulator Bcl-2 antibody, Apoptosis regulator Bcl2 antibody, AW986256 antibody, B cell CLL/lymphoma 2 antibody, B cell leukemia/lymphoma 2 antibody, Bcl-2 antibody, Bcl2 antibody, BCL2_HUMAN antibody, C430015F12Rik antibody, D630044D05Rik antibody, D830018M01Rik antibody, Leukemia/lymphoma, B-cell, 2 antibody, Oncogene B-cell leukemia 2 antibody, PPP1R50 antibody, Protein phosphatase 1, regulatory subunit 50 antibody
|Database links|| |
|Protein function|| |
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785). .
|Protein tissue specificity|| |
Expressed in a variety of tissues.
|Involvement in disease|| |
Note=A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions. .
|Protein sequence and domain|| |
Belongs to the Bcl-2 family. BH1 and BH2 domains are required for the interaction with BAX and for anti-apoptotic activity. ; The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.; The loop between motifs BH4 and BH3 is required for the interaction with NLRP1.
|Protein post-translational modifications|| |
Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity). .; Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity. .; Monoubiquitinated by PARK2, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome. .
|Protein cellular localization|| |
Mitochondrion outer membrane ; Single-pass membrane protein . Nucleus membrane ; Single-pass membrane protein . Endoplasmic reticulum membrane ; Single-pass membrane protein .
|Research area|| |
<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Tumor-SuppressorApoptosis/">Tumor Suppressor/Apoptosis</a>><a href="http://antibodyplus.com/product-tag/Bcl-2/">Bcl-2</a><br><a href="http://antibodyplus.com/product-tag/Tumor-SuppressorApoptosis/"> (View all antibody categories related to Tumor Suppressor/Apoptosis)</a>
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St John’s Laboratory Ltd.
|Product type|| |
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