Mouse Monoclonal Anti-Beclin 1 antibody (STJ99148)

$99.00$319.00

Reactivity: Human, Mouse, Rat, Monkey, Hamster
Applications: WB, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

Clear
-$50 as credit for submitting review for primary antibody
Shipping notice:
Product will be delivered in 6 to 8 Business Days

Mouse Monoclonal Anti-Beclin 1 antibody (STJ99148)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-2000; ELISA 1:10000-20000

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Anti-Beclin 1 antibody.

Alternative names:

Check alternative names for the antibody

Expand

|APG6 antibody|Bcl-2-interacting protein beclin antibody|Beclin-1 antibody|Beclin1 antibody|BECN 1 antibody|Becn1 antibody|BECN1_HUMAN antibody|Coiled coil myosin like BCL2 interacting protein antibody|Coiled-coil myosin-like BCL2-interacting protein antibody|GT197 antibody|Protein GT197 antibody|VPS 30 antibody|VPS30 antibody|Anti-Beclin 1 antibody (ab62557)
SCBT cat No: sc-10086|sc-48341|sc-48381|sc-11427|sc-10087|

Name

Anti-Beclin 1 antibody

Catalogue No.

STJ99148

Reactivity

Human, Mouse, Rat, Monkey, Hamster

Specificity

Anti-Beclin 1 antibody detects endogenous levels of Beclin 1 and does not cross-react with related proteins.

Immunogen

Purified recombinant human Beclin 1 protein fragments expressed in E.coli.

Host

Mouse

Applications

WB, ELISA

Recommended dilution

WB 1:500-2000; ELISA 1:10000-20000

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Molecular weight

55kDa

Formulation

Anti-Beclin 1 antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Anti-Beclin 1 antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

APG6 antibody, Bcl-2-interacting protein beclin antibody, Beclin-1 antibody, Beclin1 antibody, BECN 1 antibody, Becn1 antibody, BECN1_HUMAN antibody, Coiled coil myosin like BCL2 interacting protein antibody, Coiled-coil myosin-like BCL2-interacting protein antibody, GT197 antibody, Protein GT197 antibody, VPS 30 antibody, VPS30 antibody

Database links

UniProt/Swiss-Prot:Q14457

Protein function

Plays a central role in autophagy (PubMed:23184933). Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). Protects against infection by a neurovirulent strain of Sindbis virus (PubMed:9765397). May play a role in antiviral host defense. .; Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors. .

Protein tissue specificity

Ubiquitous.

Protein sequence and domain

Belongs to the beclin family. The coiled coil domain can form antiparallel homodimers and mediates dimerization with the coiled coil domains of ATG14 or UVRAG involved in the formation of PI3K complexes.

Protein post-translational modifications

Phosphorylation at Thr-119 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy (PubMed:19180116). In response to autophagic stimuli, phosphorylated at serine residues by AMPK in an ATG14-dependent manner, and this phosphorylation is critical for maximally efficient autophagy (PubMed:23878393). .; Polyubiquitinated by NEDD4, both with ‘Lys11’- and ‘Lys63’-linkages. ‘Lys’-11-linked poyubiquitination leads to degradation and is enhanced when the stabilizing interaction partner VPS34 is depleted. Deubiquitinated by USP10 and USP13, leading to stabilize the PIK3C3/VPS34-containing complexes. .; Proteolytically processed by caspases including CASP8 and CASP3; the C-terminal fragments lack autophagy-inducing capacity and are proposed to induce apoptosis. Thus the cleavage is proposed to be an determinant to switch from autophagy to apoptosis pathways affecting cellular homeostasis including viral infections and survival of tumor cells. .

Protein cellular localization

Cytoplasm . Golgi apparatus, trans-Golgi network membrane ; Peripheral membrane protein . Endosome membrane ; Peripheral membrane protein . Endoplasmic reticulum membrane ; Peripheral membrane protein . Mitochondrion membrane ; Peripheral membrane protein . Endosome . Cytoplasmic vesicle, autophagosome . Note=Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells (By similarity). .; Beclin-1-C 35 kDa: Mitochondrion . Nucleus . Cytoplasm .; Beclin-1-C 37 kDa: Mitochondrion .

Note

AntibodyPlus can customize Anti-Beclin 1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

Reviews


There are no reviews yet.

Only logged in customers who have purchased this product may leave a review

There are no reviews for this product yet.
By submitting a review, get following benefits:

1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!

view database submit reviews

There is no extra validation for this product yet.
Check other extra validated antibodies below:



Welcome to AntibodyPlus!