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Mouse Monoclonal Anti-BTK antibody (STJ99116)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-2000; ELISA 1:10000-20000
Recommended protocols: check protocols
Click or hover above images to see image description for Anti-BTK antibody.
Check alternative names for the antibodyExpand
|Agammaglobulinaemia tyrosine kinase antibody|AGMX 1 antibody|AGMX1 antibody|AT antibody|ATK antibody|B cell progenitor kinase antibody|B-cell progenitor kinase antibody|BPK antibody|Bruton agammaglobulinemia tyrosine kinase antibody|Bruton tyrosine kinase antibody|Bruton¡¯s Tyrosine Kinase antibody|Btk antibody|BTK_HUMAN antibody|dominant-negative kinase-deficient Brutons tyrosine kinase antibody|IMD 1 antibody|IMD1 antibody|MGC126261 antibody|MGC126262 antibody|OTTHUMP00000063593 antibody|PSCTK 1 antibody|PSCTK1 antibody|truncated Bruton agammaglobulinemia tyrosine kinase antibody|Tyrosine protein kinase BTK antibody|Tyrosine-protein kinase BTK antibody|tyrosine-protein kinase BTK isoform (lacking exon 14 antibody|XLA antibody|Phospho anti-BTK (Y223) antibody [EP420Y] (ab68217)
SCBT cat No: sc-81170|sc-81159|sc-1107|sc-81735|sc-81736|sc-28387|sc-1696|sc-1108|
|Catalogue No.|| |
Anti-BTK antibody detects endogenous levels of BTK and does not cross-react with related proteins.
Purified recombinant human BTK protein fragments expressed in E.coli.
|Recommended dilution|| |
WB 1:500-2000; ELISA 1:10000-20000
|Molecular weight|| |
Anti-BTK antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Anti-BTK antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Agammaglobulinaemia tyrosine kinase antibody, AGMX 1 antibody, AGMX1 antibody, AT antibody, ATK antibody, B cell progenitor kinase antibody, B-cell progenitor kinase antibody, BPK antibody, Bruton agammaglobulinemia tyrosine kinase antibody, Bruton tyrosine kinase antibody, Bruton’s Tyrosine Kinase antibody, Btk antibody, BTK_HUMAN antibody, dominant-negative kinase-deficient Brutons tyrosine kinase antibody, IMD 1 antibody, IMD1 antibody, MGC126261 antibody, MGC126262 antibody, OTTHUMP00000063593 antibody, PSCTK 1 antibody, PSCTK1 antibody, truncated Bruton agammaglobulinemia tyrosine kinase antibody, Tyrosine protein kinase BTK antibody, Tyrosine-protein kinase BTK antibody, tyrosine-protein kinase BTK isoform (lacking exon 14 antibody, XLA antibody
|Database links|| |
|Protein function|| |
Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK. BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways. Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway. The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense. Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells. Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation. BTK plays also a critical role in transcription regulation. Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes. BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B. Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR. GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression. ARID3A and NFAT are other transcriptional target of BTK. BTK is required for the formation of functional ARID3A DNA-binding complexes. There is however no evidence that BTK itself binds directly to DNA. BTK has a dual role in the regulation of apoptosis. .
|Protein tissue specificity|| |
Predominantly expressed in B-lymphocytes.
|Involvement in disease|| |
X-linked agammaglobulinemia (XLA) [MIM:300755]: Humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin. . Note=The disease is caused by mutations affecting the gene represented in this entry.; X-linked hypogammaglobulinemia and isolated growth hormone deficiency (XLA-IGHD) [MIM:307200]: In rare cases XLA is inherited together with isolated growth hormone deficiency (IGHD). Note=The disease may be caused by mutations affecting the gene represented in this entry.
|Protein sequence and domain|| |
Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily. ; Contains 1 Btk-type zinc finger. ; Contains 1 PH domain. ; Contains 1 protein kinase domain. ; Contains 1 SH2 domain. ; Contains 1 SH3 domain. The PH domain mediates the binding to inositol polyphosphate and phosphoinositides, leading to its targeting to the plasma membrane. It is extended in the BTK kinase family by a region designated the TH (Tec homology) domain, which consists of about 80 residues preceding the SH3 domain.
|Protein post-translational modifications|| |
Following B-cell receptor (BCR) engagement, translocates to the plasma membrane where it gets phosphorylated at Tyr-551 by LYN and SYK. Phosphorylation at Tyr-551 is followed by autophosphorylation of Tyr-223 which may create a docking site for a SH2 containing protein. Phosphorylation at Ser-180 by PRKCB, leads in translocation of BTK back to the cytoplasmic fraction. Phosphorylation at Ser-21 and Ser-115 creates a binding site for PIN1 at these Ser-Pro motifs, and promotes it’s recruitment. .
|Protein cellular localization|| |
Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. Note=In steady state, BTK is predominantly cytosolic. Following B-cell receptor (BCR) engagement by antigen, translocates to the plasma membrane through its PH domain. Plasma membrane localization is a critical step in the activation of BTK. A fraction of BTK also shuttles between the nucleus and the cytoplasm, and nuclear export is mediated by the nuclear export receptor CRM1.
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St John’s Laboratory Ltd.
|Product type|| |
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