Mouse Monoclonal Anti-DBC1 antibody (STJ99034)

$99.00$319.00

Reactivity: Human, Mouse, Rat, Monkey
Applications: WB, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal Anti-DBC1 antibody (STJ99034)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-2000; ELISA 1:10000-20000

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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|CCAR2 antibody|Cell cycle and apoptosis regulator 2 antibody|Cell division cycle and apoptosis regulator protein 2 antibody|DBC-1 antibody|DBC.1 antibody|DBC1 antibody|DBIRD complex subunit KIAA1967 antibody|Deleted in breast cancer 1 antibody|Deleted in breast cancer gene 1 protein antibody|K1967_HUMAN antibody|KIAA1967 antibody|NET35 antibody|p30 DBC antibody|p30 DBC protein antibody|p30DBC antibody|Anti-KIAA1967 antibody [EPR19747] (ab215852)
SCBT cat No: sc-67373|sc-166733|sc-54067|

Name

Anti-DBC1 antibody

Catalogue No.

STJ99034

Reactivity

Human, Mouse, Rat, Monkey

Specificity

Anti-DBC1 antibody detects endogenous levels of DBC1 and does not cross-react with related proteins.

Immunogen

Purified recombinant human DBC1 protein fragments expressed in E.coli

Host

Mouse

Applications

WB, ELISA

Recommended dilution

WB 1:500-2000; ELISA 1:10000-20000

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Molecular weight

130kDa

Formulation

Anti-DBC1 antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Anti-DBC1 antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

CCAR2 antibody, Cell cycle and apoptosis regulator 2 antibody, Cell division cycle and apoptosis regulator protein 2 antibody, DBC-1 antibody, DBC.1 antibody, DBC1 antibody, DBIRD complex subunit KIAA1967 antibody, Deleted in breast cancer 1 antibody, Deleted in breast cancer gene 1 protein antibody, K1967_HUMAN antibody, KIAA1967 antibody, NET35 antibody, p30 DBC antibody, p30 DBC protein antibody, p30DBC antibody

Database links

UniProt/Swiss-Prot:Q8N163

Protein function

Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity. As part of a histone H3-specific methyltransferase complex may mediate ligand-dependent transcriptional activation by nuclear hormone receptors. Plays a critical role in maintaining genomic stability and cellular integrity following UV-induced genotoxic stress. Regulates the circadian expression of the core clock components NR1D1 and ARNTL/BMAL1. Enhances the transcriptional repressor activity of NR1D1 through stabilization of NR1D1 protein levels by preventing its ubiquitination and subsequent degradation (PubMed:18235501, PubMed:18235502, PubMed:19131338, PubMed:19218236, PubMed:22446626, PubMed:23352644, PubMed:23398316). Represses the ligand-dependent transcriptional activation function of ESR2 (PubMed:20074560). Acts as a regulator of PCK1 expression and gluconeogenesis by a mechanism that involves, at least in part, both NR1D1 and SIRT1 (PubMed:24415752). Negatively regulates the deacetylase activity of HDAC3 and can alter its subcellular localization (PubMed:21030595). Positively regulates the beta-catenin pathway (canonical Wnt signaling pathway) and is required for MCC-mediated repression of the beta-catenin pathway (PubMed:24824780). Represses ligand-dependent transcriptional activation function of NR1H2 and NR1H3 and inhibits the interaction of SIRT1 with NR1H3 (PubMed:25661920). Plays an important role in tumor suppression through p53/TP53 regulation; stabilizes p53/TP53 by affecting its interaction with ubiquitin ligase MDM2 (PubMed:25732823). Represses the transcriptional activator activity of BRCA1 (PubMed:20160719). Inhibits SIRT1 in a CHEK2 and PSEM3-dependent manner and inhibits the activity of CHEK2 in vitro (PubMed:25361978). .

Protein tissue specificity

Expressed in gastric carcinoma tissue and the expression gradually increases with the progression of the carcinoma (at protein level). Expressed ubiquitously in normal tissues. Expressed in 84 to 100% of neoplastic breast, lung, and colon tissues. .

Protein post-translational modifications

ATM/ATR-mediated phosphorylation at Thr-454 upon DNA damage promotes binding to SIRT1. Phosphorylation at Thr-454 promotes its sumoylation by switching the binding partner of CCAR2 from SENP1 to PIAS3. .; Acetylation at Lys-112 and Lys-215 by KAT8 prevents inhibitory binding to SIRT1 and increases its deacetylase activity. .; Genotoxic stress induces its sumoylation and sumoylation promotes the SIRT1-CCAR2 interaction which in turn inhibits SIRT1-mediated deacetylation of p53/TP53. Sumoylation leads to transcriptional activation of p53/TP53 by sequestering SIRT1 from p53/TP53. Desumoylated by SENP1. .

Protein cellular localization

Nucleus . Cytoplasm . Note=Recruited to chromatin, post-UV irradiation. Sequestered to the cytoplasm in the presence of MCC. Translocated to the cytoplasm during UV-induced apoptosis. .

Research area

All research areas>Transcription Regulators>DBC
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize Anti-DBC1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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