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Mouse Monoclonal Anti-DBC1 antibody (STJ99034)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-2000; ELISA 1:10000-20000
Recommended protocols: check protocols
Click or hover above images to see image description for Anti-DBC1 antibody.
Check alternative names for the antibodyExpand
|CCAR2 antibody|Cell cycle and apoptosis regulator 2 antibody|Cell division cycle and apoptosis regulator protein 2 antibody|DBC-1 antibody|DBC.1 antibody|DBC1 antibody|DBIRD complex subunit KIAA1967 antibody|Deleted in breast cancer 1 antibody|Deleted in breast cancer gene 1 protein antibody|K1967_HUMAN antibody|KIAA1967 antibody|NET35 antibody|p30 DBC antibody|p30 DBC protein antibody|p30DBC antibody|Anti-KIAA1967 antibody [EPR19747] (ab215852)
SCBT cat No: sc-67373|sc-166733|sc-54067|
|Catalogue No.|| |
Human, Mouse, Rat, Monkey
Anti-DBC1 antibody detects endogenous levels of DBC1 and does not cross-react with related proteins.
Purified recombinant human DBC1 protein fragments expressed in E.coli
|Recommended dilution|| |
WB 1:500-2000; ELISA 1:10000-20000
|Molecular weight|| |
Anti-DBC1 antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Anti-DBC1 antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
CCAR2 antibody, Cell cycle and apoptosis regulator 2 antibody, Cell division cycle and apoptosis regulator protein 2 antibody, DBC-1 antibody, DBC.1 antibody, DBC1 antibody, DBIRD complex subunit KIAA1967 antibody, Deleted in breast cancer 1 antibody, Deleted in breast cancer gene 1 protein antibody, K1967_HUMAN antibody, KIAA1967 antibody, NET35 antibody, p30 DBC antibody, p30 DBC protein antibody, p30DBC antibody
|Database links|| |
|Protein function|| |
Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity. As part of a histone H3-specific methyltransferase complex may mediate ligand-dependent transcriptional activation by nuclear hormone receptors. Plays a critical role in maintaining genomic stability and cellular integrity following UV-induced genotoxic stress. Regulates the circadian expression of the core clock components NR1D1 and ARNTL/BMAL1. Enhances the transcriptional repressor activity of NR1D1 through stabilization of NR1D1 protein levels by preventing its ubiquitination and subsequent degradation (PubMed:18235501, PubMed:18235502, PubMed:19131338, PubMed:19218236, PubMed:22446626, PubMed:23352644, PubMed:23398316). Represses the ligand-dependent transcriptional activation function of ESR2 (PubMed:20074560). Acts as a regulator of PCK1 expression and gluconeogenesis by a mechanism that involves, at least in part, both NR1D1 and SIRT1 (PubMed:24415752). Negatively regulates the deacetylase activity of HDAC3 and can alter its subcellular localization (PubMed:21030595). Positively regulates the beta-catenin pathway (canonical Wnt signaling pathway) and is required for MCC-mediated repression of the beta-catenin pathway (PubMed:24824780). Represses ligand-dependent transcriptional activation function of NR1H2 and NR1H3 and inhibits the interaction of SIRT1 with NR1H3 (PubMed:25661920). Plays an important role in tumor suppression through p53/TP53 regulation; stabilizes p53/TP53 by affecting its interaction with ubiquitin ligase MDM2 (PubMed:25732823). Represses the transcriptional activator activity of BRCA1 (PubMed:20160719). Inhibits SIRT1 in a CHEK2 and PSEM3-dependent manner and inhibits the activity of CHEK2 in vitro (PubMed:25361978). .
|Protein tissue specificity|| |
Expressed in gastric carcinoma tissue and the expression gradually increases with the progression of the carcinoma (at protein level). Expressed ubiquitously in normal tissues. Expressed in 84 to 100% of neoplastic breast, lung, and colon tissues. .
|Protein post-translational modifications|| |
ATM/ATR-mediated phosphorylation at Thr-454 upon DNA damage promotes binding to SIRT1. Phosphorylation at Thr-454 promotes its sumoylation by switching the binding partner of CCAR2 from SENP1 to PIAS3. .; Acetylation at Lys-112 and Lys-215 by KAT8 prevents inhibitory binding to SIRT1 and increases its deacetylase activity. .; Genotoxic stress induces its sumoylation and sumoylation promotes the SIRT1-CCAR2 interaction which in turn inhibits SIRT1-mediated deacetylation of p53/TP53. Sumoylation leads to transcriptional activation of p53/TP53 by sequestering SIRT1 from p53/TP53. Desumoylated by SENP1. .
|Protein cellular localization|| |
Nucleus . Cytoplasm . Note=Recruited to chromatin, post-UV irradiation. Sequestered to the cytoplasm in the presence of MCC. Translocated to the cytoplasm during UV-induced apoptosis. .
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St John’s Laboratory Ltd.
|Product type|| |
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