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Mouse Monoclonal Anti-DNA-PKcs antibody (STJ99135)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-2000; ELISA 1:10000-20000
Recommended protocols: check protocols
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Check alternative names for the antibodyExpand
|DNA dependent protein kinase catalytic subunit antibody|DNA PK catalytic subunit antibody|DNA-dependent protein kinase catalytic subunit antibody|DNA-PK catalytic subunit antibody|DNA-PKcs antibody|DNAPK antibody|DNAPK catalytic subunit antibody|DNPK 1 antibody|DNPK1 antibody|Hyper radiosensitivity of murine scid mutation, complementing 1 antibody|HYRC 1 antibody|HYRC antibody|HYRC1 antibody|IMD26 antibody|p350 antibody|p460 antibody|PKRDC antibody|PRKDC antibody|PRKDC_HUMAN antibody|Protein Kinase DNA Activated Catalytic Polypeptide antibody|XRCC 7 antibody|XRCC7 antibody|Anti-DNA PKcs antibody [18-2] (ab1832)
SCBT cat No: sc-390698|sc-390849|sc-5282|sc-376207|
|Catalogue No.|| |
Anti-DNA-PKcs antibody detects endogenous levels of DNA-PKcs and does not cross-react with related proteins.
Purified recombinant human DNA-PKcs protein fragments expressed in E.coli
|Recommended dilution|| |
WB 1:500-2000; ELISA 1:10000-20000
|Molecular weight|| |
Anti-DNA-PKcs antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Anti-DNA-PKcs antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
DNA dependent protein kinase catalytic subunit antibody, DNA PK catalytic subunit antibody, DNA-dependent protein kinase catalytic subunit antibody, DNA-PK catalytic subunit antibody, DNA-PKcs antibody, DNAPK antibody, DNAPK catalytic subunit antibody, DNPK 1 antibody, DNPK1 antibody, Hyper radiosensitivity of murine scid mutation, complementing 1 antibody, HYRC 1 antibody, HYRC antibody, HYRC1 antibody, IMD26 antibody, p350 antibody, p460 antibody, PKRDC antibody, PRKDC antibody, PRKDC_HUMAN antibody, Protein Kinase DNA Activated Catalytic Polypeptide antibody, XRCC 7 antibody, XRCC7 antibody
|Database links|| |
|Protein function|| |
Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates ‘Ser-139’ of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 ‘Ser-588’ and increasing CRY1 protein stability, most likely through an indirect machanism. Interacts with CRY1 and CRY2; negatively regulates CRY1 phosphorylation. .
|Involvement in disease|| |
Immunodeficiency 26 with or without neurologic abnormalities (IMD26) [MIM:615966]: A form of severe combined immunodeficiency characterized by reduced or absent T and B cells, recurrent candidiasis, and lower respiratory tract infections. Some patients show dysmorphic features, severe growth failure, microcephaly, seizures, and impaired neurological functions. . Note=The disease is caused by mutations affecting the gene represented in this entry.
|Protein sequence and domain|| |
Belongs to the PI3/PI4-kinase family. ; Contains 1 FAT domain. ; Contains 1 FATC domain. ; Contains 2 HEAT repeats. ; Contains 1 PI3K/PI4K domain. ; Contains 3 TPR repeats.
|Protein post-translational modifications|| |
Autophosphorylated on Ser-2056, Thr-2609, Thr-2638 and Thr-2647. Ser-2056 and Thr-2609 are DNA damage-inducible phosphorylation sites (inducible with ionizing radiation, IR) dephosphorylated by PPP5C. Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair. .; S-nitrosylated by GAPDH. .; Polyubiquitinated by RNF144A, leading to proteasomal degradation. .
|Protein cellular localization|| |
Nucleus . Nucleus, nucleolus .
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St John’s Laboratory Ltd.
|Product type|| |
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