Mouse Monoclonal Anti-E-Cadherin antibody (STJ99316)

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Reactivity: Human
Applications: WB, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal Anti-E-Cadherin antibody (STJ99316)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-2000; ELISA 1:10000-20000

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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|Arc 1 antibody|CADH1_HUMAN antibody|Cadherin 1 antibody|cadherin 1 type 1 E-cadherin antibody|Cadherin1 antibody|CAM 120/80 antibody|CD 324 antibody|CD324 antibody|CD324 antigen antibody|cdh1 antibody|CDHE antibody|E-Cad/CTF3 antibody|E-cadherin antibody|ECAD antibody|Epithelial cadherin antibody|epithelial calcium dependant adhesion protein antibody|LCAM antibody|Liver cell adhesion molecule antibody|UVO antibody|Uvomorulin antibody|Anti-E Cadherin antibody [HECD-1] (ab1416)
SCBT cat No: sc-71009|sc-71008|sc-71007|sc-52327|sc-21791|sc-52328|sc-59778|sc-8426|sc-7870|sc-31021|sc-59905|sc-1500|sc-31020|sc-59780|sc-56527|sc-137991|sc-376017|

Name

Anti-E-Cadherin antibody

Catalogue No.

STJ99316

Reactivity

Human

Specificity

Anti-E-Cadherin antibody detects endogenous levels of E-Cadherin and does not cross-react with related proteins.

Immunogen

0

Host

Mouse

Applications

WB, ELISA

Recommended dilution

WB 1:500-2000; ELISA 1:10000-20000

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Molecular weight

135kDa

Formulation

Anti-E-Cadherin antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Anti-E-Cadherin antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Arc 1 antibody, CADH1_HUMAN antibody, Cadherin 1 antibody, cadherin 1 type 1 E-cadherin antibody, Cadherin1 antibody, CAM 120/80 antibody, CD 324 antibody, CD324 antibody, CD324 antigen antibody, cdh1 antibody, CDHE antibody, E-Cad/CTF3 antibody, E-cadherin antibody, ECAD antibody, Epithelial cadherin antibody, epithelial calcium dependant adhesion protein antibody, LCAM antibody, Liver cell adhesion molecule antibody, UVO antibody, Uvomorulin antibody

Database links

UniProt/Swiss-Prot:P12830

Protein function

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. .; E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production. .

Protein tissue specificity

Non-neural epithelial tissues.

Involvement in disease

Hereditary diffuse gastric cancer (HDGC) [MIM:137215]: A cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease characterized by poorly differentiated infiltrating lesions resulting in thickening of the stomach. Malignant tumors start in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. . Note=Disease susceptibility is associated with variations affecting the gene represented in this entry. Heterozygous CDH1 germline mutations are responsible for familial cases of diffuse gastric cancer. Somatic mutations has also been found in patients with sporadic diffuse gastric cancer and lobular breast cancer.; Endometrial cancer (ENDMC) [MIM:608089]: A malignancy of endometrium, the mucous lining of the uterus. Most endometrial cancers are adenocarcinomas, cancers that begin in cells that make and release mucus and other fluids. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.; Ovarian cancer (OC) [MIM:167000]: The term ovarian cancer defines malignancies originating from ovarian tissue. Although many histologic types of ovarian tumors have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease. . Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.; Breast cancer, lobular (LBC) [MIM:137215]: A type of breast cancer that begins in the milk-producing glands (lobules) of the breast. . Note=The gene represented in this entry may be involved in disease pathogenesis.

Protein sequence and domain

Contains 5 cadherin domains. Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.

Protein post-translational modifications

During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions. .; N-glycosylation at Asn-637 is essential for expression, folding and trafficking. .; Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-754. .

Protein cellular localization

Cell junction. Cell membrane; Single-pass type I membrane protein. Endosome. Golgi apparatus, trans-Golgi network. Note=Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.

Note

AntibodyPlus can customize Anti-E-Cadherin antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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