Mouse Monoclonal Anti-FEN-1 antibody (STJ99235)


Reactivity: Human, Mouse, Rat, Monkey, Hamster
Applications: WB, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal Anti-FEN-1 antibody (STJ99235)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-2000; ELISA 1:10000-20000

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody


|DNase IV antibody|FEN-1 antibody|FEN1 antibody|FEN1_HUMAN antibody|Flap endonuclease 1 antibody|Flap structure specific endonuclease 1 antibody|Flap structure-specific endonuclease 1 antibody|hFEN-1 antibody|hFEN1 antibody|Maturation factor 1 antibody|MF1 antibody|Rad2 antibody|Anti-FEN1 antibody [4E7] (ab462)
SCBT cat No: sc-56675|sc-28355|sc-8795|sc-13051|sc-8794|


Anti-FEN-1 antibody

Catalogue No.



Human, Mouse, Rat, Monkey, Hamster


Anti-FEN-1 antibody detects endogenous levels of FEN-1 and does not cross-react with related proteins.


Purified recombinant human FEN-1 protein fragments expressed in E.coli.





Recommended dilution

WB 1:500-2000; ELISA 1:10000-20000







Molecular weight



Anti-FEN-1 antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


1 mg/ml


Anti-FEN-1 antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.


-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

DNase IV antibody, FEN-1 antibody, FEN1 antibody, FEN1_HUMAN antibody, Flap endonuclease 1 antibody, Flap structure specific endonuclease 1 antibody, Flap structure-specific endonuclease 1 antibody, hFEN-1 antibody, hFEN1 antibody, Maturation factor 1 antibody, MF1 antibody, Rad2 antibody

Database links


Protein function

Structure-specific nuclease with 5′-flap endonuclease and 5′-3′ exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5′-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5′-end of a downstream Okazaki fragment. It enters the flap from the 5′-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5′-3′ exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. .

Protein sequence and domain

Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.

Protein post-translational modifications

Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300. .; Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA. .; Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA. .

Protein cellular localization

Isoform 1: Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.; Isoform FENMIT: Mitochondrion .

Research area

All research areas>Transcription Regulators>FEN
(View all antibody categories related to Transcription Regulators)


AntibodyPlus can customize Anti-FEN-1 antibody according to your requirement, including bulk product size,etc. Please contact AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.


St John’s Laboratory Ltd.

Product type

Primary antibody


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