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Mouse Monoclonal Anti-FEN-1 antibody (STJ99235)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-2000; ELISA 1:10000-20000
Recommended protocols: check protocols
Click or hover above images to see image description for Anti-FEN-1 antibody.
Check alternative names for the antibodyExpand
|DNase IV antibody|FEN-1 antibody|FEN1 antibody|FEN1_HUMAN antibody|Flap endonuclease 1 antibody|Flap structure specific endonuclease 1 antibody|Flap structure-specific endonuclease 1 antibody|hFEN-1 antibody|hFEN1 antibody|Maturation factor 1 antibody|MF1 antibody|Rad2 antibody|Anti-FEN1 antibody [4E7] (ab462)
SCBT cat No: sc-56675|sc-28355|sc-8795|sc-13051|sc-8794|
|Catalogue No.|| |
Human, Mouse, Rat, Monkey, Hamster
Anti-FEN-1 antibody detects endogenous levels of FEN-1 and does not cross-react with related proteins.
Purified recombinant human FEN-1 protein fragments expressed in E.coli.
|Recommended dilution|| |
WB 1:500-2000; ELISA 1:10000-20000
|Molecular weight|| |
Anti-FEN-1 antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Anti-FEN-1 antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
DNase IV antibody, FEN-1 antibody, FEN1 antibody, FEN1_HUMAN antibody, Flap endonuclease 1 antibody, Flap structure specific endonuclease 1 antibody, Flap structure-specific endonuclease 1 antibody, hFEN-1 antibody, hFEN1 antibody, Maturation factor 1 antibody, MF1 antibody, Rad2 antibody
|Database links|| |
|Protein function|| |
Structure-specific nuclease with 5′-flap endonuclease and 5′-3′ exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5′-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5′-end of a downstream Okazaki fragment. It enters the flap from the 5′-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5′-3′ exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. .
|Protein sequence and domain|| |
Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.
|Protein post-translational modifications|| |
Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300. .; Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA. .; Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA. .
|Protein cellular localization|| |
Isoform 1: Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.; Isoform FENMIT: Mitochondrion .
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St John’s Laboratory Ltd.
|Product type|| |
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