Mouse Monoclonal Anti-FEN-1 antibody (STJ99235)

$99.00$319.00

Reactivity: Human, Mouse, Rat, Monkey, Hamster
Applications: WB, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal Anti-FEN-1 antibody (STJ99235)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-2000; ELISA 1:10000-20000

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Anti-FEN-1 antibody.

Alternative names:

Check alternative names for the antibody

Expand

|DNase IV antibody|FEN-1 antibody|FEN1 antibody|FEN1_HUMAN antibody|Flap endonuclease 1 antibody|Flap structure specific endonuclease 1 antibody|Flap structure-specific endonuclease 1 antibody|hFEN-1 antibody|hFEN1 antibody|Maturation factor 1 antibody|MF1 antibody|Rad2 antibody|Anti-FEN1 antibody [4E7] (ab462)
SCBT cat No: sc-56675|sc-28355|sc-8795|sc-13051|sc-8794|

Name

Anti-FEN-1 antibody

Catalogue No.

STJ99235

Reactivity

Human, Mouse, Rat, Monkey, Hamster

Specificity

Anti-FEN-1 antibody detects endogenous levels of FEN-1 and does not cross-react with related proteins.

Immunogen

Purified recombinant human FEN-1 protein fragments expressed in E.coli.

Host

Mouse

Applications

WB, ELISA

Recommended dilution

WB 1:500-2000; ELISA 1:10000-20000

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Molecular weight

45kDa

Formulation

Anti-FEN-1 antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Anti-FEN-1 antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

DNase IV antibody, FEN-1 antibody, FEN1 antibody, FEN1_HUMAN antibody, Flap endonuclease 1 antibody, Flap structure specific endonuclease 1 antibody, Flap structure-specific endonuclease 1 antibody, hFEN-1 antibody, hFEN1 antibody, Maturation factor 1 antibody, MF1 antibody, Rad2 antibody

Database links

UniProt/Swiss-Prot:P39748

Protein function

Structure-specific nuclease with 5′-flap endonuclease and 5′-3′ exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5′-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5′-end of a downstream Okazaki fragment. It enters the flap from the 5′-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5′-3′ exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. .

Protein sequence and domain

Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.

Protein post-translational modifications

Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300. .; Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA. .; Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA. .

Protein cellular localization

Isoform 1: Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.; Isoform FENMIT: Mitochondrion .

Research area

All research areas>Transcription Regulators>FEN
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize Anti-FEN-1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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