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Mouse Monoclonal Anti-IkB-alpha antibody (STJ99105)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-2000; ELISA 1:10000-20000
Recommended protocols: check protocols
Click or hover above images to see image description for Anti-IkB-alpha antibody.
Check alternative names for the antibodyExpand
|IKBA antibody|MAD3 antibody|NFKBI antibody|NFKBIA antibody|Nuclear factor of kappa light chain gene enhancer in B-cells antibody|Anti-cleaved IKB alpha antibody (ab13904)
SCBT cat No: sc-71291|sc-52900|sc-23459|sc-23460|sc-25105|sc-71525|sc-52936|sc-23461|sc-56196|sc-56217|sc-52953|sc-71965|
|Catalogue No.|| |
Anti-IkB-alpha antibody detects endogenous levels of IkB-alpha (N-term) and does not cross-react with related proteins.
Purified recombinant human IkB-alpha (N-term) protein fragments expressed in E.coli.
|Recommended dilution|| |
WB 1:500-2000; ELISA 1:10000-20000
|Molecular weight|| |
Anti-IkB-alpha antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Anti-IkB-alpha antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
IKBA antibody, MAD3 antibody, NFKBI antibody, NFKBIA antibody, Nuclear factor of kappa light chain gene enhancer in B-cells antibody
|Database links|| |
|Protein function|| |
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. .
|Involvement in disease|| |
Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant (ADEDAID) [MIM:612132]: A form of ectoderma dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. This form of ectodermal dysplasia is associated with decreased production of pro-inflammatory cytokines and certain interferons, rendering patients susceptible to infection. . Note=The disease is caused by mutations affecting the gene represented in this entry.
|Protein sequence and domain|| |
Belongs to the NF-kappa-B inhibitor family. ; Contains 5 ANK repeats.
|Protein post-translational modifications|| |
Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation. .; Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability. .; Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36. .; Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.
|Protein cellular localization|| |
Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export. .
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St John’s Laboratory Ltd.
|Product type|| |
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