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Mouse Monoclonal Anti-Ku80 antibody (STJ99226)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-2000; ELISA 1:10000-20000
Recommended protocols: check protocols
Click or hover above images to see image description for Anti-Ku80 antibody.
Check alternative names for the antibodyExpand
|86 kDa subunit of Ku antigen antibody|KUB 2 antibody|KUB2 antibody|Lupus Ku autoantigen protein p86 antibody|NFIV antibody|Nuclear factor IV antibody|Thyroid lupus autoantigen antibody|Thyroid-lupus autoantigen antibody|TLAA antibody|X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining) antibody|X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) antibody|X-ray repair cross-complementing protein 5 antibody|Xray repair complementing defective repair in Chinese hamster cells 5 antibody|XRCC 5 antibody|XRCC5 antibody|XRCC5_HUMAN antibody|Anti-Ku80 antibody [5C5] (ab119935)
SCBT cat No: sc-53021|sc-135964|sc-5280|sc-1484|sc-9034|sc-33653|sc-1485|sc-56135|sc-56136|
|Catalogue No.|| |
Anti-Ku80 antibody detects endogenous levels of Ku80 and does not cross-react with related proteins.
Purified recombinant human Ku80 protein fragments expressed in E.coli
|Recommended dilution|| |
WB 1:500-2000; ELISA 1:10000-20000
|Molecular weight|| |
Anti-Ku80 antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Anti-Ku80 antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
86 kDa subunit of Ku antigen antibody, KUB 2 antibody, KUB2 antibody, Lupus Ku autoantigen protein p86 antibody, NFIV antibody, Nuclear factor IV antibody, Thyroid lupus autoantigen antibody, Thyroid-lupus autoantigen antibody, TLAA antibody, X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining) antibody, X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) antibody, X-ray repair cross-complementing protein 5 antibody, Xray repair complementing defective repair in Chinese hamster cells 5 antibody, XRCC 5 antibody, XRCC5 antibody, XRCC5_HUMAN antibody
|Database links|| |
|Protein function|| |
Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3′-5′ direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together (PubMed:12145306, PubMed:20383123, PubMed:7957065, PubMed:8621488). The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression (PubMed:20383123). The XRCC5/6 dimer probably also acts as a 5′-deoxyribose-5-phosphate lyase (5′-dRP lyase), by catalyzing the beta-elimination of the 5′ deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5′-dRP activity, and allows to ‘clean’ the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription (PubMed:8621488). .
|Protein sequence and domain|| |
Belongs to the ku80 family. ; Contains 1 Ku domain. The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
|Protein post-translational modifications|| |
Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity. .; Sumoylated. .; Ubiquitinated by RNF8 via ‘Lys-48’-linked ubiquitination following DNA damage, leading to its degradation and removal from DNA damage sites (PubMed:22266820). Ubiquitinated by RNF138, leading to remove the Ku complex from DNA breaks (PubMed:26502055). .
|Protein cellular localization|| |
Nucleus . Nucleus, nucleolus . Chromosome .
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St John’s Laboratory Ltd.
|Product type|| |
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