Mouse Monoclonal Anti-Ku80 antibody (STJ99226)


Reactivity: Human, Monkey
Applications: WB, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal Anti-Ku80 antibody (STJ99226)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-2000; ELISA 1:10000-20000

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody


|86 kDa subunit of Ku antigen antibody|KUB 2 antibody|KUB2 antibody|Lupus Ku autoantigen protein p86 antibody|NFIV antibody|Nuclear factor IV antibody|Thyroid lupus autoantigen antibody|Thyroid-lupus autoantigen antibody|TLAA antibody|X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining) antibody|X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) antibody|X-ray repair cross-complementing protein 5 antibody|Xray repair complementing defective repair in Chinese hamster cells 5 antibody|XRCC 5 antibody|XRCC5 antibody|XRCC5_HUMAN antibody|Anti-Ku80 antibody [5C5] (ab119935)
SCBT cat No: sc-53021|sc-135964|sc-5280|sc-1484|sc-9034|sc-33653|sc-1485|sc-56135|sc-56136|


Anti-Ku80 antibody

Catalogue No.



Human, Monkey


Anti-Ku80 antibody detects endogenous levels of Ku80 and does not cross-react with related proteins.


Purified recombinant human Ku80 protein fragments expressed in E.coli





Recommended dilution

WB 1:500-2000; ELISA 1:10000-20000







Molecular weight



Anti-Ku80 antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


1 mg/ml


Anti-Ku80 antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.


-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

86 kDa subunit of Ku antigen antibody, KUB 2 antibody, KUB2 antibody, Lupus Ku autoantigen protein p86 antibody, NFIV antibody, Nuclear factor IV antibody, Thyroid lupus autoantigen antibody, Thyroid-lupus autoantigen antibody, TLAA antibody, X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining) antibody, X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) antibody, X-ray repair cross-complementing protein 5 antibody, Xray repair complementing defective repair in Chinese hamster cells 5 antibody, XRCC 5 antibody, XRCC5 antibody, XRCC5_HUMAN antibody

Database links


Protein function

Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3′-5′ direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together (PubMed:12145306, PubMed:20383123, PubMed:7957065, PubMed:8621488). The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression (PubMed:20383123). The XRCC5/6 dimer probably also acts as a 5′-deoxyribose-5-phosphate lyase (5′-dRP lyase), by catalyzing the beta-elimination of the 5′ deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5′-dRP activity, and allows to ‘clean’ the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription (PubMed:8621488). .

Protein sequence and domain

Belongs to the ku80 family. ; Contains 1 Ku domain. The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.

Protein post-translational modifications

Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity. .; Sumoylated. .; Ubiquitinated by RNF8 via ‘Lys-48’-linked ubiquitination following DNA damage, leading to its degradation and removal from DNA damage sites (PubMed:22266820). Ubiquitinated by RNF138, leading to remove the Ku complex from DNA breaks (PubMed:26502055). .

Protein cellular localization

Nucleus . Nucleus, nucleolus . Chromosome .

Research area

All research areas>Tumor Suppressor/Apoptosis>Ku Lupus Antigen
(View all antibody categories related to Tumor Suppressor/Apoptosis)


AntibodyPlus can customize Anti-Ku80 antibody according to your requirement, including bulk product size,etc. Please contact AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.


St John’s Laboratory Ltd.

Product type

Primary antibody


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