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Mouse Monoclonal Anti-LIN28A antibody (STJ99177)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-2000; ELISA 1:10000-20000
Recommended protocols: check protocols
Click or hover above images to see image description for Anti-LIN28A antibody.
Check alternative names for the antibodyExpand
|AL024421 antibody|CSDD1 antibody|FLJ12457 antibody|Lin 28 antibody|Lin 28 homolog (C. elegans) antibody|Lin 28 homolog A (C. elegans) antibody|Lin 28 homolog A antibody|Lin 28 homolog antibody|Lin-28A antibody|Lin28 antibody|Lin28, C. elegans, homolog of, A antibody|LIN28A antibody|LN28A_HUMAN antibody|Protein lin-28 homolog A antibody|RNA binding protein lin 28 antibody|Tex17 antibody|ZCCHC1 antibody|Zinc finger CCHC domain containing 1 antibody|Zinc finger CCHC domain containing protein 1 antibody|Zinc finger CCHC domain-containing protein 1 antibody|Anti-Lin28 antibody (ab46020)
SCBT cat No: sc-293120|sc-54029|sc-374460|sc-54030|sc-67266|sc-54032|
|Catalogue No.|| |
Anti-LIN28A antibody detects endogenous levels of LIN28A and does not cross-react with related proteins.
Purified recombinant human LIN28A protein fragments expressed in E.coli
|Recommended dilution|| |
WB 1:500-2000; ELISA 1:10000-20000
|Molecular weight|| |
Anti-LIN28A antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Anti-LIN28A antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
AL024421 antibody, CSDD1 antibody, FLJ12457 antibody, Lin 28 antibody, Lin 28 homolog (C. elegans) antibody, Lin 28 homolog A (C. elegans) antibody, Lin 28 homolog A antibody, Lin 28 homolog antibody, Lin-28A antibody, Lin28 antibody, Lin28, C. elegans, homolog of, A antibody, LIN28A antibody, LN28A_HUMAN antibody, Protein lin-28 homolog A antibody, RNA binding protein lin 28 antibody, Tex17 antibody, ZCCHC1 antibody, Zinc finger CCHC domain containing 1 antibody, Zinc finger CCHC domain containing protein 1 antibody, Zinc finger CCHC domain-containing protein 1 antibody
|Database links|| |
|Protein function|| |
RNA-binding protein that inhibits processing of pre-let-7 miRNAs and regulates translation of mRNAs that control developmental timing, pluripotency and metabolism. Seems to recognize a common structural G-quartet (G4) feature in its miRNA and mRNA targets (Probable). 'Translational enhancer' that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in mRNA stabilization. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Suppressor of microRNA (miRNA) biogenesis, including that of let-7, miR107, miR-143 and miR-200c. Specifically binds the miRNA precursors (pre-miRNAs), recognizing an 5'-GGAG-3' motif found in pre-miRNA terminal loop, and recruits ZCCHC11/TUT4 uridylyltransferase. This results in the terminal uridylation of target pre-miRNAs. Uridylated pre-miRNAs fail to be processed by Dicer and undergo degradation. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state by preventing let-7-mediated differentiation of embryonic stem cells (PubMed:18951094, PubMed:19703396, PubMed:22118463, PubMed:22898984). Localized to the periendoplasmic reticulum area, binds to a large number of spliced mRNAs and inhibits the translation of mRNAs destined for the ER, reducing the synthesis of transmembrane proteins, ER or Golgi lumen proteins, and secretory proteins. Binds to and enhances the translation of mRNAs for several metabolic enzymes, such as PFKP, PDHA1 or SDHA, increasing glycolysis and oxidative phosphorylation. Which, with the let-7 repression may enhance tissue repair in adult tissue (By similarity). .
|Protein tissue specificity|| |
Expressed in embryonic stem cells, placenta and testis. Tends to be up-regulated in HER2-overexpressing breast tumors. .
|Protein sequence and domain|| |
Belongs to the lin-28 family. ; Contains 2 CCHC-type zinc fingers. ; Contains 1 CSD (cold-shock) domain. The CSD domain is required for function in muscle differentiation. ; The CCHC zinc fingers interact with the GGAG motif at the 3' end of let-7 miRNAs precursors, more generally they bind the 5'-NGNNG-3' consensus motif with micromolar affinity. The CSD domain recognizes the loop at the 5' end. The flexible linker allows accommodating variable sequences and lengths among let-7 family members.
|Protein cellular localization|| |
Cytoplasm . Nucleus, nucleolus. Rough endoplasmic reticulum . Note=Predominantly cytoplasmic (PubMed:22118463). Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules. .
|Research area|| |
<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Transcription-Regulators/">Transcription Regulators</a>><a href="http://antibodyplus.com/product-tag/LIN-28/">LIN-28</a><br><a href="http://antibodyplus.com/product-tag/Transcription-Regulators/"> (View all antibody categories related to Transcription Regulators)</a>
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St John’s Laboratory Ltd.
|Product type|| |
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