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Mouse Monoclonal Anti-MKK3/6 antibody (STJ99282)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-2000; ELISA 1:10000-20000
Recommended protocols: check protocols
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Check alternative names for the antibodyExpand
|Dual specificity mitogen activated protein kinase kinase 6 antibody|Dual specificity mitogen-activated protein kinase kinase 6 antibody|MAP kinase kinase 6 antibody|MAP2K6 antibody|MAPK/ERK kinase 6 antibody|MAPKK 6 antibody|MAPKK6 antibody|MEK 6 antibody|MEK6 antibody|Mitogen Activated Protein Kinase Kinase 6 antibody|MKK 6 antibody|MKK6 antibody|MP2K6_HUMAN antibody|PRKMK6 antibody|Protein kinase mitogen activated kinase 6 antibody|protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6) antibody|SAPK kinase 3 antibody|SAPKK-3 antibody|SAPKK3 antibody|SKK3 antibody|Stress-activated protein kinase kinase 3 antibody|Anti-MEK3 + MEK6 antibody [EPR17340] (ab200831)
SCBT cat No: sc-166746|sc-166727|sc-166728|sc-1991|sc-1992|sc-136337|
|Catalogue No.|| |
Anti-MKK3/6 antibody detects endogenous levels of MKK3 and MKK6 and does not cross-react with related proteins.
Purified recombinant human MKK6 protein fragments expressed in E.coli.
|Recommended dilution|| |
WB 1:500-2000; ELISA 1:10000-20000
|Molecular weight|| |
Anti-MKK3/6 antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Anti-MKK3/6 antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Dual specificity mitogen activated protein kinase kinase 6 antibody, Dual specificity mitogen-activated protein kinase kinase 6 antibody, MAP kinase kinase 6 antibody, MAP2K6 antibody, MAPK/ERK kinase 6 antibody, MAPKK 6 antibody, MAPKK6 antibody, MEK 6 antibody, MEK6 antibody, Mitogen Activated Protein Kinase Kinase 6 antibody, MKK 6 antibody, MKK6 antibody, MP2K6_HUMAN antibody, PRKMK6 antibody, Protein kinase mitogen activated kinase 6 antibody, protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6) antibody, SAPK kinase 3 antibody, SAPKK-3 antibody, SAPKK3 antibody, SKK3 antibody, Stress-activated protein kinase kinase 3 antibody
|Database links|| |
|Protein function|| |
Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. With MAP3K3/MKK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinases p38 MAPK11, MAPK12, MAPK13 and MAPK14 and plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. Especially, MAP2K3/MKK3 and MAP2K6/MKK6 are both essential for the activation of MAPK11 and MAPK13 induced by environmental stress, whereas MAP2K6/MKK6 is the major MAPK11 activator in response to TNF. MAP2K6/MKK6 also phosphorylates and activates PAK6. The p38 MAP kinase signal transduction pathway leads to direct activation of transcription factors. Nuclear targets of p38 MAP kinase include the transcription factors ATF2 and ELK1. Within the p38 MAPK signal transduction pathway, MAP3K6/MKK6 mediates phosphorylation of STAT4 through MAPK14 activation, and is therefore required for STAT4 activation and STAT4-regulated gene expression in response to IL-12 stimulation. The pathway is also crucial for IL-6-induced SOCS3 expression and down-regulation of IL-6-mediated gene induction; and for IFNG-dependent gene transcription. Has a role in osteoclast differentiation through NF-kappa-B transactivation by TNFSF11, and in endochondral ossification and since SOX9 is another likely downstream target of the p38 MAPK pathway. MAP2K6/MKK6 mediates apoptotic cell death in thymocytes. Acts also as a regulator for melanocytes dendricity, through the modulation of Rho family GTPases. .
|Protein tissue specificity|| |
Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas. .
|Protein sequence and domain|| |
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. ; Contains 1 protein kinase domain. The DVD domain (residues 311-334) contains a conserved docking site and is found in the mammalian MAP kinase kinases (MAP2Ks). The DVD sites bind to their specific upstream MAP kinase kinase kinases (MAP3Ks) and are essential for activation. ; The D domain (residues 4-19) contains a conserved docking site and is required for the binding to MAPK substrates.
|Protein post-translational modifications|| |
Weakly autophosphorylated. Phosphorylated at Ser-207 and Thr-211 by the majority of M3Ks, such as MAP3K5/ASK1, MAP3K1/MEKK1, MAP3K2/MEKK2, MAP3K3/MEKK3, MAP3K4/MEKK4, MAP3K7/TAK1, MAP3K11/MLK3 and MAP3K17/TAOK2. .; Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. .
|Protein cellular localization|| |
Nucleus . Cytoplasm . Cytoplasm, cytoskeleton . Note=Binds to microtubules.
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St John’s Laboratory Ltd.
|Product type|| |
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