Mouse Monoclonal Anti-p27 KIP1 antibody (STJ99255)

$99.00$319.00

Reactivity: Mouse
Applications: WB, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal Anti-p27 KIP1 antibody (STJ99255)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-2000; ELISA 1:10000-20000

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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|AA408329 antibody|AI843786 antibody|Cdki1b antibody|CDKN 1B antibody|CDKN 4 antibody|CDKN1B antibody|CDKN4 antibody|CDN1B_HUMAN antibody|Cyclin Dependent Kinase Inhibitor 1B antibody|Cyclin dependent kinase inhibitor p27 antibody|Cyclin-dependent kinase inhibitor 1B (p27, Kip1) antibody|Cyclin-dependent kinase inhibitor 1B antibody|Cyclin-dependent kinase inhibitor p27 antibody|Cyclin-dependent kinase inhibitor p27 Kip1 antibody|KIP 1 antibody|KIP1 antibody|MEN1B antibody|MEN4 antibody|OTTHUMP00000195098 antibody|OTTHUMP00000195099 antibody|p27 antibody|p27 Kip1 antibody|P27-like cyclin-dependent kinase inhibitor antibody|p27Kip1 antibody|Anti-p27 KIP 1 antibody [Y236] (ab32034)
SCBT cat No: sc-393380|sc-528|sc-393305|sc-56338|sc-1641|sc-776|sc-527|sc-56454|sc-53906|sc-53871|sc-6359|sc-9962|

Name

Anti-p27 KIP1 antibody

Catalogue No.

STJ99255

Reactivity

Mouse

Specificity

Anti-p27 KIP1 antibody detects endogenous levels of p27 Kip1 and does not cross-react with related proteins.

Immunogen

Purified recombinant human p27 Kip1 protein fragments expressed in E.coli.

Host

Mouse

Applications

WB, ELISA

Recommended dilution

WB 1:500-2000; ELISA 1:10000-20000

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Molecular weight

27kDa

Formulation

Anti-p27 KIP1 antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Anti-p27 KIP1 antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

AA408329 antibody, AI843786 antibody, Cdki1b antibody, CDKN 1B antibody, CDKN 4 antibody, CDKN1B antibody, CDKN4 antibody, CDN1B_HUMAN antibody, Cyclin Dependent Kinase Inhibitor 1B antibody, Cyclin dependent kinase inhibitor p27 antibody, Cyclin-dependent kinase inhibitor 1B (p27, Kip1) antibody, Cyclin-dependent kinase inhibitor 1B antibody, Cyclin-dependent kinase inhibitor p27 antibody, Cyclin-dependent kinase inhibitor p27 Kip1 antibody, KIP 1 antibody, KIP1 antibody, MEN1B antibody, MEN4 antibody, OTTHUMP00000195098 antibody, OTTHUMP00000195099 antibody, p27 antibody, p27 Kip1 antibody, P27-like cyclin-dependent kinase inhibitor antibody, p27Kip1 antibody

Database links

UniProt/Swiss-Prot:P46527

Protein function

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. .

Protein tissue specificity

Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.

Involvement in disease

Multiple endocrine neoplasia 4 (MEN4) [MIM:610755]: Multiple endocrine neoplasia (MEN) syndromes are inherited cancer syndromes of the thyroid. MEN4 is a MEN-like syndrome with a phenotypic overlap of both MEN1 and MEN2. . Note=The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

Belongs to the CDI family. A peptide sequence containing only AA 28-79 retains substantial Kip1 cyclin A/CDK2 inhibitory activity.

Protein post-translational modifications

Phosphorylated; phosphorylation occurs on serine, threonine and tyrosine residues. Phosphorylation on Ser-10 is the major site of phosphorylation in resting cells, takes place at the G(0)-G(1) phase and leads to protein stability. Phosphorylation on other sites is greatly enhanced by mitogens, growth factors, cMYC and in certain cancer cell lines. The phosphorylated form found in the cytoplasm is inactivate. Phosphorylation on Thr-198 is required for interaction with 14-3-3 proteins. Phosphorylation on Thr-187, by CDK1 and CDK2 leads to protein ubiquitination and proteasomal degradation. Tyrosine phosphorylation promotes this process. Phosphorylation by PKB/AKT1 can be suppressed by LY294002, an inhibitor of the catalytic subunit of PI3K. Phosphorylation on Tyr-88 and Tyr-89 has no effect on binding CDK2, but is required for binding CDK4. Dephosphorylated on tyrosine residues by G-CSF. .; Ubiquitinated; in the cytoplasm by the KPC complex (composed of RNF123/KPC1 and UBAC1/KPC2) and, in the nucleus, by SCF(SKP2). The latter requires prior phosphorylation on Thr-187. Ubiquitinated; by a TRIM21-containing SCF(SKP2)-like complex; leads to its degradation. .; Subject to degradation in the lysosome. Interaction with SNX6 promotes lysosomal degradation (By similarity). .

Protein cellular localization

Nucleus. Cytoplasm. Endosome . Note=Nuclear and cytoplasmic in quiescent cells. AKT- or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6; this leads to lysosomal degradation (By similarity). .

Note

AntibodyPlus can customize Anti-p27 KIP1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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