Mouse Monoclonal Anti-POU5F1/OCT4 antibody (STJ99042)

$99.00$319.00

Reactivity: Mouse
Applications: WB, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal Anti-POU5F1/OCT4 antibody (STJ99042)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-2000; ELISA 1:10000-20000

Recommended protocols: check protocols

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SCBT cat No:

Name

Anti-POU5F1/OCT4 antibody

Catalogue No.

STJ99042

Reactivity

Mouse

Specificity

Anti-POU5F1/OCT4 antibody detects endogenous levels of POU5F1/OCT4 and does not cross-react with related proteins.

Immunogen

Purified recombinant mouse POU5F1/OCT4 protein fragments expressed in E.coli.

Host

Mouse

Applications

WB, ELISA

Recommended dilution

WB 1:500-2000; ELISA 1:10000-20000

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG2b

Molecular weight

45kDa

Formulation

Anti-POU5F1/OCT4 antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Anti-POU5F1/OCT4 antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Database links

UniProt/Swiss-Prot:Q01860

Protein function

Transcription factor that binds to the octamer motif (5′-ATTTGCAT-3′). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. .

Protein tissue specificity

Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. .

Protein sequence and domain

Belongs to the POU transcription factor family. Class-5 subfamily. ; Contains 1 homeobox DNA-binding domain. ; Contains 1 POU-specific domain. The POU-specific domain mediates interaction with PKM.

Protein post-translational modifications

Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression (By similarity). .; Ubiquitinated; undergoes ‘Lys-63’-linked polyubiquitination by WWP2 leading to proteasomal degradation. .; ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription. .

Protein cellular localization

Cytoplasm. Nucleus. Note=Expressed in a diffuse and slightly punctuate pattern.

Note

AntibodyPlus can customize Anti-POU5F1/OCT4 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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