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Mouse Monoclonal Anti-POU5F1/OCT4 antibody (STJ99042)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-2000; ELISA 1:10000-20000
Recommended protocols: check protocols
Click or hover above images to see image description for Anti-POU5F1/OCT4 antibody.
Check alternative names for the antibodyExpand
SCBT cat No:
|Catalogue No.|| |
Anti-POU5F1/OCT4 antibody detects endogenous levels of POU5F1/OCT4 and does not cross-react with related proteins.
Purified recombinant mouse POU5F1/OCT4 protein fragments expressed in E.coli.
|Recommended dilution|| |
WB 1:500-2000; ELISA 1:10000-20000
|Molecular weight|| |
Anti-POU5F1/OCT4 antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Anti-POU5F1/OCT4 antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Database links|| |
|Protein function|| |
Transcription factor that binds to the octamer motif (5′-ATTTGCAT-3′). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. .
|Protein tissue specificity|| |
Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. .
|Protein sequence and domain|| |
Belongs to the POU transcription factor family. Class-5 subfamily. ; Contains 1 homeobox DNA-binding domain. ; Contains 1 POU-specific domain. The POU-specific domain mediates interaction with PKM.
|Protein post-translational modifications|| |
Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression (By similarity). .; Ubiquitinated; undergoes ‘Lys-63’-linked polyubiquitination by WWP2 leading to proteasomal degradation. .; ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription. .
|Protein cellular localization|| |
Cytoplasm. Nucleus. Note=Expressed in a diffuse and slightly punctuate pattern.
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St John’s Laboratory Ltd.
|Product type|| |
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