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Mouse Monoclonal Anti-PRMT4/CARM1 antibody (STJ99037)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-2000; ELISA 1:10000-20000
Recommended protocols: check protocols
Click or hover above images to see image description for Anti-PRMT4/CARM1 antibody.
Check alternative names for the antibodyExpand
|carm1 antibody|CARM1_HUMAN antibody|Coactivator associated arginine methyltransferase 1 antibody|Coactivator-associated arginine methyltransferase 1 antibody|Histone arginine methyltransferase CARM 1 antibody|Histone arginine methyltransferase CARM1 antibody|Histone-arginine methyltransferase CARM1 antibody|PRMT 4 antibody|PRMT4 antibody|Protein arginine methyltransferase antibody|Protein arginine N methyltransferase 4 antibody|Protein arginine N-methyltransferase 4 antibody|Anti-PRMT4 antibody (ab128851)
SCBT cat No: sc-390656|sc-393381|sc-33176|sc-5421|sc-5418|
|Catalogue No.|| |
Anti-PRMT4/CARM1 antibody detects endogenous levels of PRMT4/CARM1 and does not cross-react with related proteins.
Purified recombinant human PRMT4/CARM1 protein fragments expressed in E.coli.
|Recommended dilution|| |
WB 1:500-2000; ELISA 1:10000-20000
|Molecular weight|| |
Anti-PRMT4/CARM1 antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Anti-PRMT4/CARM1 antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
carm1 antibody, CARM1_HUMAN antibody, Coactivator associated arginine methyltransferase 1 antibody, Coactivator-associated arginine methyltransferase 1 antibody, Histone arginine methyltransferase CARM 1 antibody, Histone arginine methyltransferase CARM1 antibody, Histone-arginine methyltransferase CARM1 antibody, PRMT 4 antibody, PRMT4 antibody, Protein arginine methyltransferase antibody, Protein arginine N methyltransferase 4 antibody, Protein arginine N-methyltransferase 4 antibody
|Database links|| |
|Protein function|| |
Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs. .
|Protein tissue specificity|| |
Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia. .
|Protein sequence and domain|| |
Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. ; Contains 1 SAM-dependent MTase PRMT-type domain.
|Protein post-translational modifications|| |
Auto-methylated on Arg-550. Methylation enhances transcription coactivator activity. Methylation is required for its role in the regulation of pre-mRNA alternative splicing (By similarity). .; Phosphorylation at Ser-216 interferes with S-adenosyl-L-methionine binding and strongly reduces methyltransferase activity (By similarity). Phosphorylation at Ser-216 is strongly increased during mitosis, and decreases rapidly to a very low, basal level after entry into the G1 phase of the cell cycle. Phosphorylation at Ser-216 may promote location in the cytosol. .
|Protein cellular localization|| |
Nucleus. Cytoplasm. Note=Mainly nuclear during the G1, S and G2 phases of the cell cycle. Cytoplasmic during mitosis, after breakup of the nuclear membrane.
|Research area|| |
<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Transcription-Regulators/">Transcription Regulators</a>><a href="http://antibodyplus.com/product-tag/CARM/">CARM</a><br><a href="http://antibodyplus.com/product-tag/Transcription-Regulators/"> (View all antibody categories related to Transcription Regulators)</a>
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St John’s Laboratory Ltd.
|Product type|| |
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