Mouse Monoclonal Anti-RPA32/RPA2 antibody (STJ99210)


Reactivity: Human
Applications: WB, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal Anti-RPA32/RPA2 antibody (STJ99210)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-2000; ELISA 1:10000-20000

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody


|60S acidic ribosomal protein P1 antibody|AA409079 antibody|AI325195 antibody|AU020965 antibody|ik:tdsubc_2g1 antibody|M(2)21C antibody|MGC137236 antibody|OTTHUMP00000004008 antibody|p32 antibody|p34 antibody|RCJMB04_6d17 replication protein A2, 32kDa antibody|REPA2 antibody|Replication factor A protein 2 antibody|Replication protein A 32 kDa subunit antibody|Replication protein A 32kDa subunit antibody|Replication protein A 34 kDa subunit antibody|Replication protein A antibody|Replication Protein A2 (32kDa) antibody|Replication protein A2 antibody|Replication protein A2, 32kDa antibody|RF-A protein 2 antibody|Rf-A2 antibody|RFA antibody|RFA2_HUMAN antibody|RP-A p32 antibody|RP-A p34 antibody|RP21C antibody|RPA 2 antibody|RPA 32 antibody|RPA antibody|Rpa2 antibody|RPA32 antibody|RPA34 antibody|RpLP1 antibody|RpP2 antibody|xx:tdsubc_2g1 antibody|zgc:109822 antibody|Anti-RPA32/RPA2 antibody [9H8] (ab2175)
SCBT cat No: sc-56770|sc-271578|sc-14692|sc-166886|sc-28709|sc-53496|sc-14691|sc-56594|


Anti-RPA32/RPA2 antibody

Catalogue No.





Anti-RPA32/RPA2 antibody detects endogenous levels of RPA32/RPA2 and does not cross-react with related proteins.


Purified recombinant human RPA32/RPA2 protein fragments expressed in E.coli.











Molecular weight



Anti-RPA32/RPA2 antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


1 mg/ml


Anti-RPA32/RPA2 antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.


-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

60S acidic ribosomal protein P1 antibody, AA409079 antibody, AI325195 antibody, AU020965 antibody, ik:tdsubc_2g1 antibody, M(2)21C antibody, MGC137236 antibody, OTTHUMP00000004008 antibody, p32 antibody, p34 antibody, RCJMB04_6d17 replication protein A2, 32kDa antibody, REPA2 antibody, Replication factor A protein 2 antibody, Replication protein A 32 kDa subunit antibody, Replication protein A 32kDa subunit antibody, Replication protein A 34 kDa subunit antibody, Replication protein A antibody, Replication Protein A2 (32kDa) antibody, Replication protein A2 antibody, Replication protein A2, 32kDa antibody, RF-A protein 2 antibody, Rf-A2 antibody, RFA antibody, RFA2_HUMAN antibody, RP-A p32 antibody, RP-A p34 antibody, RP21C antibody, RPA 2 antibody, RPA 32 antibody, RPA antibody, Rpa2 antibody, RPA32 antibody, RPA34 antibody, RpLP1 antibody, RpP2 antibody, xx:tdsubc_2g1 antibody, zgc:109822 antibody

Protein function

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. .

Protein sequence and domain

Belongs to the replication factor A protein 2 family. ; Contains 1 OB DNA-binding domain.

Protein post-translational modifications

Differentially phosphorylated throughout the cell cycle, becoming phosphorylated at the G1-S transition and dephosphorylated in late mitosis. Mainly phosphorylated at Ser-23 and Ser-29, by cyclin A-CDK2 and cyclin B-CDK1, respectively during DNA replication and mitosis. Dephosphorylation may require the serine/threonine-protein phosphatase 4. Phosphorylation at Ser-23 and Ser-29 is a prerequisite for further phosphorylation. Becomes hyperphosphorylated on additional residues including Ser-4, Ser-8, Thr-21 and Ser-33 in response to DNA damage. Hyperphosphorylation is mediated by ATM, ATR and PRKDC. Primarily recruited to DNA repair nuclear foci as a hypophosphorylated form it undergoes subsequent hyperphosphorylation, catalyzed by ATR. Hyperphosphorylation is required for RAD51 recruitment to chromatin and efficient DNA repair. Phosphorylation at Thr-21 depends upon RFWD3 presence. .; DNA damage-induced 'Lys-63'-linked polyubiquitination by PRPF19 mediates ATRIP recruitment to the RPA complex at sites of DNA damage and activation of ATR. .

Protein cellular localization

Nucleus . Nucleus, PML body . Note=Redistributes to discrete nuclear foci upon DNA damage in an ATR-dependent manner. .

Research area

<a href="">All research areas</a>><a href="">Transcription Regulators</a>><a href="">Replication Protein A</a><br><a href=""&gt; (View all antibody categories related to Transcription Regulators)</a>


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St John’s Laboratory Ltd.

Product type

Primary antibody


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