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Mouse Monoclonal Anti-RPA70 antibody (STJ99205)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-2000; ELISA 1:10000-20000
Recommended protocols: check protocols
Click or hover above images to see image description for Anti-RPA70 antibody.
Check alternative names for the antibodyExpand
|Dmrpa1 antibody|Drosophila Replication Protein A antibody|DRPA antibody|HSSB antibody|Human single stranded DNA binding protein antibody|MST075 antibody|MSTP075 antibody|p70 antibody|REPA1 antibody|Replication factor A antibody|Replication factor A protein 1 antibody|Replication protein A 70 kDa DNA-binding subunit antibody|Replication protein A 70kDa DNA binding subunit antibody|Replication protein A1 70kDa antibody|Replication protein A1 antibody|RF A antibody|RF-A protein 1 antibody|RFA antibody|RFA1_HUMAN antibody|RP A antibody|RP-A p70 antibody|RPA 70 antibody|RPA antibody|rpa1 antibody|Single stranded binding protein 70 antibody|Single-stranded DNA-binding protein antibody|Anti-RPA70 antibody [EPR3472] (ab79398)
SCBT cat No: sc-166023|sc-28304|sc-14696|sc-25376|sc-48425|sc-53497|sc-46504|
|Catalogue No.|| |
Human, Monkey, Mouse, Rat
Anti-RPA70 antibody detects endogenous levels of RPA70 and does not cross-react with related proteins.
Purified recombinant human RPA70 protein fragments expressed in E.coli.
|Recommended dilution|| |
WB 1:500-2000; ELISA 1:10000-20000
|Molecular weight|| |
Anti-RPA70 antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Anti-RPA70 antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Dmrpa1 antibody, Drosophila Replication Protein A antibody, DRPA antibody, HSSB antibody, Human single stranded DNA binding protein antibody, MST075 antibody, MSTP075 antibody, p70 antibody, REPA1 antibody, Replication factor A antibody, Replication factor A protein 1 antibody, Replication protein A 70 kDa DNA-binding subunit antibody, Replication protein A 70kDa DNA binding subunit antibody, Replication protein A1 70kDa antibody, Replication protein A1 antibody, RF A antibody, RF-A protein 1 antibody, RFA antibody, RFA1_HUMAN antibody, RP A antibody, RP-A p70 antibody, RPA 70 antibody, RPA antibody, rpa1 antibody, Single stranded binding protein 70 antibody, Single-stranded DNA-binding protein antibody
|Database links|| |
|Protein function|| |
As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism (PubMed:27723720, PubMed:27723717). Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response (PubMed:24332808). It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage (PubMed:17765923). Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair (PubMed:7697716). Plays also a role in base excision repair (BER) probably through interaction with UNG (PubMed:9765279). Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance (PubMed:17959650). As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange (PubMed:19996105). .
|Protein sequence and domain|| |
Belongs to the replication factor A protein 1 family. ; Contains 1 OB DNA-binding domain.
|Protein post-translational modifications|| |
DNA damage-induced ‘Lys-63’-linked polyubiquitination by PRPF19 mediates ATRIP recruitment to the RPA complex at sites of DNA damage and activation of ATR. .; Sumoylated on lysine residues Lys-449 and Lys-577, with Lys-449 being the major site. Sumoylation promotes recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombination. Desumoylated by SENP6. .
|Protein cellular localization|| |
Nucleus . Nucleus, PML body . Note=Enriched in PML bodies in cells displaying alternative lengthening of their telomeres. .
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St John’s Laboratory Ltd.
|Product type|| |
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