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Mouse Monoclonal Anti-Smad2 antibody (STJ99174)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-2000; ELISA 1:10000-20000
Recommended protocols: check protocols
Click or hover above images to see image description for Anti-Smad2 antibody.
Check alternative names for the antibodyExpand
|Drosophila, homolog of, MADR2 antibody|SMAD 2 antibody|SMAD family member 2 antibody|SMAD, mothers against DPP homolog 2 antibody|SMAD2 antibody|SMAD2_HUMAN antibody|Anti-Smad2 antibody [EP784Y] (ab40855)
SCBT cat No: sc-101801|sc-11769|sc-130218|sc-514317|
|Catalogue No.|| |
Anti-Smad2 antibody detects endogenous levels of Smad2 and does not cross-react with related proteins.
Purified recombinant human Smad2 protein fragments expressed in E.coli.
|Recommended dilution|| |
WB 1:500-2000; ELISA 1:10000-20000
|Molecular weight|| |
Anti-Smad2 antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Anti-Smad2 antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Drosophila, homolog of, MADR2 antibody, SMAD 2 antibody, SMAD family member 2 antibody, SMAD, mothers against DPP homolog 2 antibody, SMAD2 antibody, SMAD2_HUMAN antibody
|Database links|| |
|Protein function|| |
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. .
|Protein tissue specificity|| |
Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta. .
|Protein sequence and domain|| |
Belongs to the dwarfin/SMAD family. ; Contains 1 MH1 (MAD homology 1) domain. ; Contains 1 MH2 (MAD homology 2) domain.
|Protein post-translational modifications|| |
Phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. TGF-beta-induced Ser-465/467 phosphorylation declines progressively in a KMT5A-dependent manner. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. Phosphorylated by PDPK1. .; In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation. Monoubiquitinated, leading to prevent DNA-binding (By similarity). Deubiquitination by USP15 alleviates inhibition and promotes activation of TGF-beta target genes (PubMed:21947082). Ubiquitinated by RNF111, leading to its degradation: only SMAD2 proteins that are ‘in use’ are targeted by RNF111, RNF111 playing a key role in activating SMAD2 and regulating its turnover (By similarity). .; Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo. Acetylation in the nucleus by EP300 is enhanced by TGF-beta. .
|Protein cellular localization|| |
Cytoplasm . Nucleus . Note=Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:9865696). On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081). .
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St John’s Laboratory Ltd.
|Product type|| |
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