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Mouse Monoclonal Anti-Wnt3a antibody (STJ99281)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-2000; ELISA 1:10000-20000
Recommended protocols: check protocols
Click or hover above images to see image description for Anti-Wnt3a antibody.
Check alternative names for the antibodyExpand
SCBT cat No:
|Catalogue No.|| |
Anti-Wnt3a antibody detects endogenous levels of Wnt3a and Wnt3.
Purified recombinant Wnt3a protein fragments expressed in E.coli.
|Recommended dilution|| |
WB 1:500-2000; ELISA 1:10000-20000
|Molecular weight|| |
Anti-Wnt3a antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Anti-Wnt3a antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Database links|| |
|Protein function|| |
Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
|Protein tissue specificity|| |
Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate.
|Protein sequence and domain|| |
Belongs to the Wnt family.
|Protein post-translational modifications|| |
Palmitoleylation by PORCN is required for efficient binding to frizzled receptors. Palmitoleylation is required for proper trafficking to cell surface, vacuolar acidification is critical to release palmitoleylated WNT3A from WLS in secretory vesicles (PubMed:20826466, PubMed:21244856, PubMed:24292069). Depalmitoleylated by NOTUM, leading to inhibit Wnt signaling pathway, possibly by promoting disulfide bond formation and oligomerization (PubMed:25731175). .; Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of WNT3A. .; Disulfide bonds have critical and distinct roles in secretion and activity. Loss of each conserved cysteine in WNT3A results in high molecular weight oxidized Wnt oligomers, which are formed through inter-Wnt disulfide bonding. .
|Protein cellular localization|| |
Secreted, extracellular space, extracellular matrix.
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St John’s Laboratory Ltd.
|Product type|| |
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