Mouse Monoclonal Aquaporin 4 antibody [4H1] (STJ96964)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal Aquaporin 4 antibody [4H1] (STJ96964)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IF

Recommended dilution: WB 1:1000; IF 1:100-200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Aquaporin 4 Monoclonal Antibody.

Alternative names:

Check alternative names for the antibody

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AQP4 antibody,|AQP 4 antibody|AQP-4 antibody|AQP4 antibody|AQP4_HUMAN antibody|Aquaporin type 4 antibody|Aquaporin-4 antibody|Aquaporin4 antibody|HMIWC 2 antibody|HMIWC2 antibody|Mercurial insensitive water channel antibody|Mercurial-insensitive water channel antibody|MGC22454 antibody|MIWC antibody|WCH 4 antibody|WCH4 antibody|Anti-Aquaporin 4 antibody (ab46182)
SCBT cat No: sc-32739|sc-390488|sc-9888|sc-390636|sc-9887|sc-20812|

Name

Aquaporin 4 Monoclonal Antibody

Catalogue No.

STJ96964

Reactivity

Human, Mouse, Rat

Specificity

The antibody detects endogenous Aquaporin 4 proteins.

Immunogen

Synthetic Peptide

Host

Mouse

Applications

WB, IF

Recommended dilution

WB 1:1000; IF 1:100-200

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

Aquaporin 4 Antibody was tube-contained. PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Purification

Aquaporin 4 Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Aquaporin-4 antibody, AQP-4 antibody, Mercurial-insensitive water channel antibody, MIWC antibody, WCH4 antibody

Database links

Human UniProt/Swiss-Prot:P55087;Mouse UniPort/Swiss-Prot: P55088;Rat UniProt/Swiss-Port: A0A0G2K4I1;Human Entrez Gene: 361;Mouse Entrez Gene: 11829;Rat Entrez Gene: Rn.90091

Protein names

Aquaporin-4 , AQP-4 , Mercurial-insensitive water channel , MIWC , WCH4

Protein function

Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.

Protein tissue specificity

Brain – muscle >> heart, kidney, lung, and trachea.

Protein sequence and domain

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). / Belongs to the MIP/aquaporin (TC 1.A.8) family. [View classification]

Protein post-translational modifications

Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamats increases conductance by 40% (By similarity).

Protein cellular localization

Membrane; Multi-pass membrane protein

Research area

All research areas>Channel Proteins>AQP
(View all antibody categories related to Channel Proteins)

Note

AntibodyPlus can customize Aquaporin 4 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human appendix tissue

1: Aquaporin 4 Monoclonal Antibody(4H1)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human appendix tissue

1: Aquaporin 4 Monoclonal Antibody(4H1)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human liver cancer tissue

1: Aquaporin 4 Monoclonal Antibody(4H1)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human liver cancer tissue

1: Aquaporin 4 Monoclonal Antibody(4H1)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse brain tissue

1: Aquaporin 4 Monoclonal Antibody(4H1)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse brain tissue

1: Aquaporin 4 Monoclonal Antibody(4H1)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human liver cancer tissue

1: Aquaporin 4 Monoclonal Antibody(4H1) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach tissue

1: Aquaporin 4 Monoclonal Antibody(4H1) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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