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Mouse Monoclonal ARK-1 antibody [4G10] (STJ97850)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:200-1:1000; ELISA 1:10000
Recommended protocols: check protocols
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Check alternative names for the antibodyExpand
AURKA antibody, AIK antibody, AIRK1 antibody, ARK1 antibody, AURA antibody, AYK1 antibody, BTAK antibody, IAK1 antibody, STK15 antibody, STK6 antibody,|AIK antibody|AYK antibody|AYK1 antibody|Breast tumor-amplified kinase antibody|BTAK antibody|IAK1 antibody|Serine/threonine-protein kinase 12 antibody|Serine/threonine-protein kinase 15 antibody|Serine/threonine-protein kinase 5 antibody|Serine/threonine-protein kinase 6 antibody|Serine/threonine-protein kinase aurora-A antibody|Serine/threonine-protein kinase aurora-B antibody|STK-1 antibody|STK1 antibody|STK12 antibody|STK15 antibody|STK5 antibody|STK6 antibody|Anti-Aurora A antibody [35C1] (ab13824)
SCBT cat No: sc-56881|sc-514374|sc-398814|sc-25425|sc-14318|sc-65987|sc-393357|
ARK-1 Monoclonal Antibody
|Catalogue No.|| |
Human, Rat, Monkey
ARK-1 Monoclonal Antibody detects endogenous levels of ARK-1 protein.
Purified recombinant fragment of human ARK-1 expressed in E Coli
WB, IHC, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:200-1:1000; ELISA 1:10000
ARK-1 Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.
ARK-1 Antibody was purified using affinity purification.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Aurora kinase A antibody, Aurora 2 antibody, Aurora/IPL1-related kinase 1 antibody, ARK-1 antibody, Aurora-related kinase 1 antibody, hARK1 antibody, Breast tumor-amplified kinase antibody, Serine/threonine-protein kinase 15 antibody, Serine/threonine-protein kinase 6 antibody, Serine/threonine-protein kinase aurora-A antibody
|Protein names|| |
Aurora kinase A , Aurora 2 , Aurora/IPL1-related kinase 1 , ARK-1 , Aurora-related kinase 1 , hARK1 , Breast tumor-amplified kinase , Serine/threonine-protein kinase 15 , Serine/threonine-protein kinase 6 , Serine/threonine-protein kinase aurora-A
|Protein function|| |
Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis. / ATP + a protein = ADP + a phosphoprotein. / Activation of CDK1, appears to be an upstream event of AURKA activation. Phosphatase inhibitor-2 (PPP1R2) and TPX2 act also as activators. Inactivated by the G2 checkpoint. Inhibited by GADD45A and p53/TP53, and through dephosphorylation by protein phosphatase type 1 (PP1). MLN8054 is also a potent and selective inhibitor. Activated during the early phase of cilia disassembly in the presence of PIFO.
|Protein tissue specificity|| |
Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
|Protein sequence and domain|| |
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily. / Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Thr-288 can be phosphorylated by several kinases, including PAK and PKA. Protein phosphatase type 1 (PP1) binds AURKA and inhibits its activity by dephosphorylating Thr-288 during mitosis. Phosphorylation at Ser-342 decreases the kinase activity. PPP2CA controls degradation by dephosphorylating Ser-51 at the end of mitosis. / Ubiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL7) during mitosis, leading to its degradation by the proteasome. Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome.
|Protein cellular localization|| |
Cytoplasm > cytoskeleton > microtubule organizing center > centrosome / Cytoplasm > cytoskeleton > spindle pole / Cytoplasm > cytoskeleton > cilium basal body / Cytoplasm > cytoskeleton > microtubule organizing center > centrosome > centriole
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St John’s Laboratory Ltd.
|Product type|| |
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